略歴

1993 年	京都大学理学部卒業
1995 年	京都大学大学院理学研究科修士課程修了
1998 年	京都大学大学院理学研究科博士後期課程修了
1998 年	博士（理学）取得
1998 年	日本学術振興会特別研究員（京都大学ウイルス研究所）
1999 年	日本学術振興会特別研究員（基礎生物学研究所）
2001 年	北海道大学大学院薬学研究科　生体機能化学分野　助手
2005 年	北海道大学大学院薬学研究科　生体機能化学分野　助教授
2007 年	北海道大学大学院薬学研究院　生体機能化学研究室　准教授
2008 年	北海道大学大学院薬学研究院　生化学研究室　教授
2017 年	北海道大学教育研究評議会評議員
2021 年	北海道大学大学院薬学研究院　研究院長（薬学部長）

学術賞

　平成 17 年度　日本生化学会奨励賞
　　「スフィンゴ脂質シグナリングと膜動態の解明」

　平成 26 年度　北海道大学研究総長賞奨励賞

　平成 27 年度　第6回 JSC Award（セラミド研究会）

学会

セラミド研究会　会長

学部担当授業科目

生化学 I，生化学 II

研究業績

179. Takahashi T, Turker S, Sassa T, Akcapinar GB, Yararbas K, Susguna S, Iseri SAU, Kihara A, Akcakaya NH. (2022) Hypomyelinating spastic dyskinesia and ichthyosis caused by a homozygous splice site mutation leading to exon skipping in ELOVL1. Brain Dev, 44, 391-400.
178. Otsuka K, Sawai M, Kihara A. (2022) Formation of fatty alcohols―components of meibum lipids―by the fatty acyl-CoA reductase FAR2 is essential for dry eye prevention. FASEB J, 36, e22216.
177. Nojiri K, Fudetani S, Arai A, Kitamura T, Sassa T, Kihara A. (2021) Impaired skin barrier function due to reduced ω-O-acylceramide levels in a mouse model of Sjögren-Larsson syndrome. Mol Cell Biol, 41, e0035221.
176. 木原章雄. (2021) 皮膚透過性バリアの形成に重要な脂質セラミドの産生機構. 進化する皮膚科学　～機能研究・臨床・評価・製品開発の最前線～ , p47-55.
175. Sassa T, Imai Y, Kihara A, Yamanishi K. (2021) Protein-bound ceramide levels in the epidermis of transglutaminase 1-deficient mice. J Dermatol, 48, 1799-1801.
174. Takeda K, Yano H, Yamada K, Kihara A. (2021) Amlexanox enhances the antitumor effect of anti-PD-1 antibody. Biochem Biophys Res Commun, 560, 1-6.
173. Sawai M, Watanabe K, Tanaka K, Kinoshita W, Otsuka K, Miyamoto M, Sassa T, Kihara A. (2021) Diverse meibum lipids produced by Awat1 and Awat2 are important for stabilizing tear film and protecting the ocular surface. iScience, 24, 102478.
（プレスリリース：北大，AMED）
172. Watanabe K, Yoshida M, Okumura T, Sassa T, Kihara A, Uchiyama A. (2021) Improvement of evaporative dry eye with meibomian gland dysfunction in model mice by treatment with ophthalmic solution containing mineral oil. Transl Vis Sci Technol, 10, 21.
171. Goto H, Miyamoto M, Kihara A. (2021) Direct uptake of sphingosine-1-phosphate (S1P) independent of phospholipid phosphatases. J Biol Chem, 296, 100605.
170. Yamamoto M, Sassa T, Kyono Y, Uemura H, Kugo M, Hayashi H, Imai Y, Yamanishi K, Kihara A. (2021) Comprehensive stratum corneum ceramide profiling reveals reduced acylceramides in ichthyosis patient with CERS3 mutations. J Dermatol, 48, 447-456.
169. Nohara T, Ohno Y, Kihara A. (2021) Impaired production of the skin barrier lipid acylceramide by CYP4F22 ichthyosis mutations. J Dermatol Sci, 101, 69-71.
168. Tanno H, Sassa T, Sawai M, Kihara A. (2021) Production of branched-chain very-long-chain fatty acids by fatty acid elongases and their tissue distribution in mammals. Biochim Biophys Acta Mol Cell Biol Lipids, 1866, 158842.
167. Mori K, Obara T, Seki N, Miyamoto M, Naganuma T, Kitamura T, Kihara A. (2020) Catalytic residues, substrate specificity, and role in carbon starvation of the 2-hydroxy FA dioxygenase Mpo1 in yeast. J Lipid Res, 61, 1104-1114.
166. Kawana M, Miyamoto M, Ohno Y, Kihara A. (2020) Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC-MS/MS. J Lipid Res, 61, 884-895.
165. Miyamoto M, Sassa T, Sawai M, Kihara A. (2020) Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease. eLife, 9, e53582.
（プレスリリース：北大，AMED）（JST サイエンスポータル）
164. Wang ZN, Kawabori M, Higashikawa K, Yasui H, Kuge Y, Ohno Y, Kihara A, Midori YA, Houkin K. (2020) FTY720 protects against ischemia-reperfusion injury by preventing the redistribution of tight junction proteins and decreases inflammation in the sub-acute phase in an experimental stroke model. Transl Stroke Res, in press.
163. Yamamoto H, Hattori M, Chamulitrat W, Ohno Y, Kihara A. (2020) Skin permeability barrier formation by the ichthyosis-causative gene FATP4 through formation of the barrier lipid ω-O-acylceramide. Proc Natl Acad Sci USA, 117, 2914-2922.
（プレスリリース：北大，AMED）
162. Jojima K, Edagawa M, Sawai M, Ohno Y, Kihara A. (2020) Biosynthesis of the anti-lipid-microdomain sphingoid base 4,14-sphingadiene by the ceramide desaturase FADS3. FASEB J, 34, 3318-3335.
161. Kawaguchi M, Sassa T, Kidokoro H, Nakata T, Kato K, Muramatsu H, Okuno Y, Yamamoto H, Kaname T, Kihara A, Natsume J. (2020) Novel biallelic FA2H mutations in a Japanese boy with fatty acid hydroxylase-associated neurodegeneration. Brain Dev, 42, 217-221.
160. Obara K, Kotani T, Nakatogawa H, Kihara A, Kamura T. (2020) N-glycosylation of Rim21 at an unconventional site fine-tunes its behavior in the plasma membrane. Cell Struct Funct, 45, 1-8.
159. Miyamoto M, Itoh N, Sawai M, Sassa T, Kihara A. (2019) Severe skin permeability barrier dysfunction in knockout mice deficient in a fatty acid ω-hydroxylase crucial to acylceramide production. J Invest Dermatol, 140, 319-326.
158. Isokawa M, Sassa T, Hattori S, Miyakawa T, Kihara A. (2019) Reduced chain length in myelin sphingolipids and poorer motor coordination in mice deficient in the fatty acid elongase Elovl1. FASEB BioAdv, 1, 747-759.
157. 木原章雄 (2019) 小胞体における新たな脂肪酸α酸化経路. 酵素工学ニュース, 82, 22-25. Review
156. 木原章雄 (2019) スフィンゴ脂質クオリティによる生体防御. The Lipid, 30, 28-34. Review
155. 木原章雄 (2019) 極長鎖脂質による表皮および涙液における透過性バリア形成. 医学のあゆみ, 269, 983-988. Review
154. 木原章雄 (2019) スフィンゴ脂質恒常性維持のためのセラミド分解経路. セラミド研究の新展開　～基礎から応用へ～ (セラミド研究会編，食品化学新聞社), 32-43. Review
153. Kinugasa Y, Hirano Y, Sawai M, Ohno Y, Shindo T, Asakawa H, Chikashige Y, Shibata S, Kihara A, Haraguchi T, Hiraoka Y. (2019) Very-long-chain fatty acid elongase Elo2 rescues lethal defects associated with loss of the nuclear barrier function. J Cell Sci, 229021.
152. Hirabayashi T, Murakami M, Kihara A. (2019) The role of PNPLA1 in ω-O-acylceramide synthesis and skin barrier function. Biochim Biophys Acta, 1864, 869-879. Review
151. Seki N, Mori K, Kitamura T, Miyamoto M, Kihara A. (2019) Yeast Mpo1 is a novel dioxygenase that catalyzes the α-oxidation of a 2-hydroxy fatty acid in an Fe²⁺-dependent manner. Mol Cell Biol, 39, e00428-18.
150. Mueller N, Sassa T, Morales-Gonzalez S, Schneider J, Salchow DJ, Seelow D, Knierim E, Stenzel W, Kihara A, Schuelke M. (2019) De novo mutation in ELOVL1 causes ichthyosis, acanthosis nigricans, hypomyelination, spastic paraplegia, high frequency deafness, and optic atrophy. J Med Genet, 56, 164-175.
149. Kanetake T, Sassa T, Nojiri K, Sawai M, Hattori S, Miyakawa T, Kitamura T, Kihara A. (2019) Neural symptoms in a gene knockout mouse model of Sjögren-Larsson syndrome are associated with a decrease in 2-hydroxygalactosylceramide. FASEB J, 33, 928-941.
148. Ohno Y, Nara A, Nakamichi S, Kihara A. (2018) Molecular mechanism of the ichthyosis pathology of Chanarin-Dorfman syndrome: stimulation of PNPLA1-catalyzed ω-O-acylceramide production by ABHD5. J Dermatol Sci, 92, 245-253.
147. Swamy M, Murai Y, Ohno Y, Jojima K, Kihara A, Mitsutake S, Igarashi Y, Yu J, Yao M, Suga Y, Anetai M, Monde K. (2018) Structure-inspired design of a sphingolipid mimic sphingosine-1-phosphate receptor agonist from a naturally occurring sphingomyelin synthase inhibitor. ChemComm, 54, 12758-12761.
146. 木原章雄 (2018) パルミトイル化. 生体の科学, 69, 490-491. Review
145. Edagawa M, Sawai M, Ohno Y, Kihara A. (2018) Widespread tissue distribution and synthetic pathway of polyunsaturated C24:2 sphingolipids in mammals. Biochim Biophys Acta, 1863, 1441-1448.
144. 木原章雄 (2018) スフィンゴ脂質長鎖塩基の代謝と脂肪酸α酸化. Plant Morphol, 30, 5-14. Review
143. Asakura T, Ishii M, Namkoong H, Suzuki S, Kagawa S, Yagi K, Komiya T, Hashimoto T, Okamori S, Kamata H, Tasaka S, Kihara A, Hegab AE, Hasegawa N, Betsuyaku T. (2018) Sphingosine 1-phosphate receptor modulator ONO-4641 dtimulates CD11b⁺Gr-1⁺ cell expansion and inhibits lymphocyte infiltration in the lungs to ameliorate murine pulmonary emphysema Mucosal Immunol, 11, 1606-1620.
142. 木原章雄 (2018) スフィンゴ脂質代謝と疾患制御. 実験医学, 36, 46-52. Review
141. 村上誠, 木原章雄 (2018) 脂質による皮膚バリア形成と疾患制御. 実験医学, 36, 160-167. Review
140. 木原章雄 (2018) 鎖長の違いによる脂肪酸クオリティ ― 極長鎖・超長鎖脂肪酸の産生機構と病態. 医学のあゆみ, 264, 966-971. Review
139. Sassa T, Tadaki M, Kiyonari H, Kihara A. (2018) Very long-chain tear film lipids produced by fatty acid elongase ELOVL1 prevent dry eye disease in mice. FASEB J, 32, 2966-2978.
（プレスリリース：北大，AMED）
138. Piña F, Yagisawa F, Obara K, Gregerson JD, Kihara A, Niwa M. (2018) Sphingolipids activate the endoplasmic reticulum stress surveillance pathway. J Cell Biol, 217, 495-505.
137. Honda Y, Kitamura T, Naganuma T, Abe T, Ohno Y, Sassa T, Kihara A. (2018) Decreased skin barrier lipid acylceramide and differentiation-dependent gene expression in ichthyosis gene Nipal4 knockout mice. J Invest Dermatol, 138, 741-749.
136. Sawai M, Uchida Y, Ohno Y, Miyamoto M, Nishioka C, Itohara S, Sassa T, Kihara A. (2017) The 3-hydroxyacyl-CoA dehydratases HACD1 and HACD2 exhibit functional redundancy and are active in a wide range of fatty acid elongation pathways. J Biol Chem, 292, 15538-15551.
135. Takeichi T, Torrelo A, Lee JYW, Ohno Y, Lozano ML, Kihara A, Liu L, Yasuda Y, Ishikawa J, Murase T, Rodrigo AB, Fernandez-Crehuet P, Toi Y, Mellerio J, Rivera J, Vicente V, Kelsell DP, Nishimura Y, Okuno Y, Kojima D, Ogawa Y, Sugiura K, Simpson MA, McLean WHI, Akiyama M, McGrath JA. (2017) Biallelic mutations in KDSR disrupt ceramide synthesis and result in a spectrum of keratinization disorders associated with thrombocytopenia. J Invest Dermatol, 137, 2344-2353.
134. Ghanegolmohammadi F, Yoshida M, Ohnuki S, Sukegawa Y, Okada H, Obara K, Kihara A, Suzuki K, Kojima T, Yachie N, Hirata D, Ohya Y. (2017) Systematic analysis of Ca²⁺ homoeostasis in Saccharomyces cerevisiae based on chemical-genetic interaction profiles. Mol Biol Cell, 28, 3415-3427.
133. Kitamura T, Seki N, Kihara A. (2017) The phytosphingosine degradation pathway includes fatty acid α-oxidation reactions in the endoplasmic reticulum. Proc Natl Acad Sci USA, 114, E2616-E2623.
132. Ohno Y, Kamiyama N, Nakamichi S, Kihara A. (2017) PNPLA1 is a transacylase essential for the generation of the skin barrier lipid ω-O-acylceramide. Nat Commun, 8, 14610.
（プレスリリース：北大，AMED）
（MedicalResearch.comで紹介）
131. Wakabayashi M, Yoshioka T, Higashino K, Numata Y, Igarashi Y, Kihara A. (2017) Decreases in 15-lipoxygenase metabolites in Olmsted syndrome model rats. J Dermatol Sci, 85, 186-196.
130. Yoshinaga T, Uwabe K, Naito S, Higashino K, Nakano T, Numata Y, Kihara A. (2016) AM251 suppresses epithelial-mesenchymal transition of renal tubular epithelial cells. PLoS One, 11, e0167848.
129. Obara K, Kihara A. (2017) The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane. Biochem J, 474, 51-63.
128. Payet LA, Leroux M, Willison JC, Kihara A, Pelosi L, Pierrel F. (2016) Mechanistic details of early steps in coenzyme Q biosynthesis pathway in yeast. Cell Chem Biol, 23, 1241-1250.
127. Makuta H, Obara K, Kihara A. (2017) Loop 5 region is important for the activity of the long-chain base transporter Rsb1. J Biochem, 161, 207-213.
126. Narita T, Naganuma T, Sase Y, Kihara A. (2016) Long-chain bases of sphingolipids are transported into cells via the acyl-CoA synthetases. Sci Rep, 6, 25469.
125. Kihara A. (2016) Synthesis and degradation pathways, functions, and pathology of ceramides and epidermal acylceramides. Prog Lipid Res, 63, 50-69. Review
124. Naganuma T, Takagi S, Kanetake T, Kitamura T, Hattori S, Miyakawa T, Sassa T, Kihara A. (2016) Disruption of the Sjögren-Larsson syndrome gene Aldh3a2 in mice increases keratinocyte growth and retards skin barrier recovery. J Biol Chem, 291,11676-11688.
123. Sassa T, Hirayama T, Kihara A. (2016) Enzyme activities of the ceramide synthases CERS2-6 are regulated by phosphorylation in the C-terminal region. J Biol Chem, 291, 7477-7487.
122. 木原章雄 (2016) 明らかとなったスフィンゴ脂質の代謝経路：フィトスフィンゴシン代謝による奇数鎖脂肪酸の産生. 化学と生物, 54, 75-76. Review
121. Klionsky DJ, et al. (2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autphagy, 12, 1-222. Review
120. Yamamoto S, Yako Y, Fujioka Y, Kajita M, Kameyama T, Kon S, Ishikawa S, Ohba Y, Ohno Y, Kihara A, Fujita Y (2016) A role of the sphingosine-1-phosphate (S1P)- S1P receptor 2 pathway in epithelial defense against cancer (EDAC). Mol Biol Cell, 27, 491-499.
119. Kihara A (2015) Sphingolipid metabolism via sphingosine 1-phosphate. Bioactive lipid mediators: current reviews and protocols (Yokomizo, T., and Murakami, M. eds.), Springer Japan, Tokyo. pp127-138. Review
118. Nishino K, Obara K, Kihara A (2015) The C-terminal cytosolic region of Rim21 senses alterations in plasma membrane lipid composition: insights into sensing mechanisms for plasma membrane lipid asymmetry. J Biol Chem, 290, 30797-30805.
117. Sugimoto M, Shimizu Y, Yoshioka T, Wakabayashi M, Tanaka Y, Higashino K, Numata Y, Sakai S, Kihara A, Igarashi Y, Kuge Y (2015) Histological analyses by matrix-assisted laser desorption/ionization-imaging mass spectrometry reveal differential localization of sphingomyelin molecular species regulated by particular ceramide synthase in mouse brains. Biochim Biophys Acta, 1851, 1554-1565.
116. 木原章雄 (2015) スフィンゴ脂質の多様性と多機能性. 実験医学, 33, 50-56. Review
115. 木原章雄 (2015) セラミドによる皮膚バリア形成. 実験医学, 33, 117-122. Review
114. Ohno Y, Nakamichi S, Ohkuni A, Kamiyama N, Naoe A, Tsujimura H, Yokose U, Sugiura K, Ishikawa J, Akiyama M, Kihara A (2015) Essential role of the cytochrome P450 CYP4F22 in the production of acylceramide, the key lipid for skin permeability barrier formation. Proc Natl Acad Sci USA, 112, 7707-7712.
（プレスリリースの内容はこちら）
113. Blondelle J, Ohno Y, Gache V, Guyot S, Storck S, Blanchard-Gutton N, Barthélémy I, Walmsley G, Rahier A, Gadin S, Maurer M, Guillaud L, Prola A, Ferry A, Aubin-Houzelstein G, Demarquoy J, Relaix F, Piercy R J, Blot S, Kihara A, Tiret L, Pilot-Storck F (2015) HACD1, a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth. J Mol Cell Biol, 7, 429-440.
112. Yamauchi S, Obara K, Uchibori K, Kamimura A, Azumi K, Kihara A (2015) Opt2 mediates the exposure of phospholipids during cellular adaptation to altered lipid asymmetry. J Cell Sci, 128, 61-69.
111. Kitamura T, Takagi S, Naganuma T, Kihara A (2015) Mouse aldehyde dehydrogenase ALDH3B2 is localized to lipid droplets via two C-terminal tryptophan residues and lipid modification. Biochem J, 465, 79-87.
（USACO のホームページで紹介）
110. Kondo N, Ohno Y, Yamagata M, Obara T, Seki N, Kitamura T, Naganuma T, Kihara A (2014) Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acids. Nat Commun, 5, 5338. Corrigendum: (2015) 6, 6815
109. Ianiri G, Abhyankar R, Kihara A, Idnurm A (2014) Phs1 and the synthesis of very long chain fatty acids are required for ballistospore formation. PLos One, 9, e105147.
108. Wakashima T, Abe K, Kihara A (2014) Dual functions of the trans-2-enoyl-CoA reductase TER in the sphingosine-1-phosphate metabolic pathway and in fatty acid elongation. J Biol Chem, 289, 24736-24748.
107. Obara K, Kihara A (2014) Signaling events of the Rim101 pathway occur at the plasma membrane in a ubiquitination-dependent manner. Mol Cell Biol, 34, 3525-3534.
106. Naganuma T, Kihara A (2014) Two modes of regulation of the fatty acid elongase ELOVL6 by the 3-ketoacyl-CoA reductase KAR in the fatty acid elongation cycle. PLos One, 9, e101823.
105. 木原章雄 (2014) スフィンゴ脂質の代謝と関連疾患. 医学のあゆみ, 248, 1112-1118. Review
104. Sassa T, Kihara A (2014) Metabolism of very long-chain fatty acids: genes and pathophysiology. Biomol Ther, 22, 83-92. Review
103. Sassa T, Wakashima T, Ohno Y, Kihara A (2014) Lorenzo's oil inhibits ELOVL1 and lowers the level of sphingomyelin with a saturated very long-chain fatty acid. J Lipid Res, 55, 524-530.
（ASBMB のブログ「WILD TYPES」で紹介）
102. Ito K, Morimoto J, Kihara A, Matsui Y, Kurotaki D, Kanayama M, Simmons S, Ishii M, Sheppard D, Takaoka A, Uede T (2014) Integrin α9 on lymphatic endothelial cell regulates lymphocyte egress. Proc Natl Acad Sci USA, 111, 3080-3085.
101. Abe K, Ohno Y, Sassa T, Taguchi R, Caliskan M, Ober C, Kihara A (2013) Mutation for non-syndromic mental retardation in the trans-2-enoyl-CoA reductase TER gene involved in fatty acid elongation impairs the enzyme activity and stability leading to change in sphingolipid profile. J Biol Chem, 288, 36741-36749.
100. Ohkuni A, Ohno Y, Kihara A (2013) Identification of acyl-CoA synthetases involved in the mammalian sphingosine 1-phosphate metabolic pathway. Biochem Biophys Res Commun, 442, 195-201.
99. Kihara A (2014) Sphingosine 1-phosphate is a key metabolite linking sphingolipids to glycerophospholipids. Biochim Biophys Acta, 1841, 766-772. Review
98. Muhammad E, Reish O, Ohno Y, Scheetz T, DeLuca A, Searby C, Regev M, Benyamini L, Fellig Y, Kihara A, Sheffield VC, Parvari R (2013) Congenital myopathy is caused by mutation of HACD1. Hum Mol Genet, 22, 5229-5236.
97. 木原章雄 (2013) スフィンゴシン1-リン酸の代謝経路の全容と中間代謝体としての重要性. 生化学, 85, 553-557. Review
96. 木原章雄 (2013) スフィンゴシン1-リン酸の代謝経路. 遺伝子医学 MOOK, 24, 175-179. Review
95. Kitamura T, Naganuma T, Abe K, Nakahara K, Ohno Y, Kihara A (2013) Substrate specificity, plasma membrane localization, and lipid modification of the aldehyde dehydrogenase ALDH3B1. Biochim Biophys Acta, 1831, 1395-1401.
94. Mizutani Y, Sun H, Ohno Y, Sassa T, Wakashima T, Obara M, Yuyama K, Kihara A, Igarashi Y (2013) Cooperative synthesis of ultra long-chain fatty acid and ceramide during keratinocyte differentiation. PLoS One, 8, e67317.
93. Sassa T, Ohno Y, Suzuki S, Nomura T, Nishioka C, Kashiwagi T, Hirayama T, Akiyama M, Taguchi R, Shimizu H, Itohara S, Kihara A (2013) Impaired epidermal permeability barrier in mice lacking the Elovl1 gene responsible for very long-chain fatty acid production. Mol Cell Biol, 33, 2787-2796.
（医薬系情報サイト Global Medical Discovery の Key Scientific Article として紹介。）
92. Yamagata M, Obara K, Kihara A (2013) Unperverted synthesis of complex sphingolipids is essential for cell survival under nitrogen starvation. Genes Cells, 18, 650-659.
91. Yazawa T, Naganuma T, Yamagata M, Kihara A (2013) Identification of residues important for the catalysis, structure maintenance, and substrate specificity of yeast 3-hydroxyacyl-CoA dehydratase Phs1. FEBS Lett, 587, 804-809.
90. Obara K, Kojima R, Kihara A (2013) Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids. J Lipid Res, 54, 831-842.
（医薬系情報サイト MDLinx で紹介。）
89. 木原章雄 (2012) 脂質の多彩な役割. 知のフロンティア (北海道大学), 2, 86-87. 研究室の紹介
88. 木原章雄 (2012) オートファジーと小胞輸送におけるスフィンゴ脂質の役割. 生体の科学, 63, 482-485. Review
87. Obara K, Yamamoto H, Kihara A (2012) Membrane protein Rim21 plays a central role in sensing ambient pH in Saccharomyces cerevisiae. J Biol Chem, 287, 38473-38481.
86. Ohno Y, Kashio A, Ogata R, Ishitomi A, Yamazaki Y, Kihara A (2012) Analysis of substrate specificity of human DHHC protein acyltransferases using a yeast expression system. Mol Biol Cell, 23, 4543-4551.
85. Kihara A (2012) Very long-chain fatty acids: elongation, physiology, and related disorders. J Biochem, 152, 387-395. Review
84. Nakahara K, Ohkuni A, Kitamura T, Abe K, Naganuma T, Ohno Y, Zoeller RA, Kihara A (2012) Sjögren-Larsson syndrome gene encodes a hexadecenal dehydrogenase of the sphingosine 1-phosphate degradation pathway. Mol Cell, 46, 461-471.
（日本語詳細がライフサイエンス新着論文レビューに掲載。）
83. Tanigawa M, Kihara A, Terashima M, Takahara T, Maeda T (2012) Sphingolipids regulate the yeast high osmolarity-responsive HOG pathway. Mol Cell Biol, 32, 2861-2870.
82. Klinosky DJ, et al. (2012) Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, 8, 445-544. Review
81. Sassa T, Suto S, Okayasu Y, Kihara A (2012) A shift in sphingolipid composition from C24 to C16 increases susceptibility to apoptosis in HeLa cells. Biochim Biophys Acta, 1821, 1031-1037.
80. 木原章雄，五十嵐靖之 (2012) 生理活性脂質とは. 臨床検査, 56, 107-116. Review
79. Lakkaraju AK, Abrami L, Lemmin T, Blaskovic S, Kunz B, Kihara A, Dal Peraro M, van der Goot FG (2012) Palmitoylated calnexin is a key component of the ribosome-translocon complex. EMBO J, 31, 1823-1835.
78. Nakagawa N, Kato M, Takahashi Y, Shimazaki KI, Tamura K, Tokuji Y, Kihara A, Imai H (2012) Degradation of long-chain base 1-phosphate (LCBP) in Arabidopsis: functional characterization of LCBP phosphatase involved in the dehydration stress response. J Plant Res, 125, 439-449.
77. 大野祐介，木原章雄 (2011) 極長鎖脂肪酸伸長とセラミド合成. ここまできたセラミド研究最前線　セラミド -基礎と応用- (セラミド研究会編，食品化学新聞社), 43-49. Review
76. Naganuma T, Sato Y, Sassa T, Ohno Y, Kihara A (2011) Biochemical characterization of the very long-chain fatty acid elongase ELOVL7. FEBS Lett, 585, 3337-3341.
75. Yamagata M, Obara K, Kihara A (2011) Sphingolipid synthesis is involved in autophagy in Saccharomyces cerevisiae. Biochem Biophys Res Commun, 410, 786-791.
74. Okuda A, Naganuma T, Ohno Y, Abe K, Yamagata M, Igarashi Y, Kihara A (2010) Hetero-oligomeric interactions of an ELOVL4 mutant protein: implications in the molecular mechanism of Stargardt-3 macular dystrophy. Mol Vis, 16, 2438-2445.
73. Ohno Y, Suto S, Yamanaka M, Mizutani Y, Mitsutake S, Igarashi Y, Sassa T, Kihara A (2010) ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis. Proc Natl Acad Sci USA, 107, 18439-18444.
72. Konishi H, Okuda A, Ohno Y, Kihara A (2010) Characterization of HACD1 K64Q mutant found in arrhythmogenic right ventricular dysplasia patients. J Biochem, 148, 617-622.
71. 木原章雄 (2010) 脂肪酸の多彩な代謝，生理機能と関連疾患. 生化学, 82, 591-605. Review
70. 木原章雄 (2009)「北から南から」生化学, 81, 626. 研究室の紹介
69. Ohno Y, Ito A, Ogata R, Hiraga Y, Igarashi Y, Kihara A (2009) Palmitoylation of the sphingosine 1-phosphate receptor S1P₁ is involved in its signaling functions and internalization. Genes Cells, 14, 911-923.
68. Mizutani Y, Mitsutake S, Tsuji K, Kihara A, Igarashi Y (2009) Ceramide biosynthesis in keratinocyte and its role in skin function. Biochimie, 91, 784-790. Review
67. E S, Lai Y, Tsukahara R, Chen C, Fujiwara Y, Yue J, Yu J, Guo H, Kihara A, Tigyi G, Lin F (2009) The LPA₂ receptor-mediated supramolecular complex formation regulates its anti-apoptotic effect. J Biol Chem, 284, 14558-14571.
66. Balan L, Foltyn VN, Zehl M, Dumin E, Dikopoltsev E, Knoh D, Ohno Y, Kihara A, Jensen ON, Radzishevsky IS, Wolosker H (2009) Feedback inactivation of D-serine synthesis by NMDA receptor-elicited translocation of serine racemase to the membrane. Proc Natl Acad Sci USA,106, 7589-7594.
65. Hara-Yokoyama M, Terasawa K, Kihara A, Kim J W, Par CS, Hirabayashi Y, Igarashi Y, Yanagishita M (2008) Sphingosine kinase 2 inhibitor accelerates Fas-mediated cell death progression in A20/2J cells. Open Bioactive Com J, 1, 22-27.
64. Yamanaka M, Anada Y, Igarashi Y, Kihara A (2008) A splicing isoform of LPP1, LPP1a, exhibits high phosphatase activity toward FTY720 phosphate. Biochem Biophys Res Commun, 375, 675-679.
63. Mizutani Y, Kihara A, Chiba H, Tojo H, Igarashi Y (2008) Synthesis of 2-hydroxy-ceramide by ceramide synthase family members: enzymatic basis for the preference of fatty acid chain length in cultured cell models. J Lipid Res, 49, 2356-2364.
62. Ikeda M, Kanao Y, Yamanaka M, Sakuraba H, Mizutani Y, Igarashi Y, Kihara A (2008) Characterization of four mammalian 3-hydroxyacyl-CoA dehydratases involved in very long-chain fatty acid synthesis. FEBS Lett, 582, 2435-2440.
61. Jin YX, Shi LH, Yoo HS, Lee YM, Kihara A, Igarashi Y, So HY, Yim YH (2008) A sphingosine kinase activity assay using directe infusion electrospray ionization tandem mass spectrometry. Anal Biochem, 380, 35-40.
60. Kihara A, Igarashi Y (2008) Production and release of sphingosine 1-phosphate and the phosphorylated form of the immunomodulator FTY720. Biochim Biophys Acta, 1781, 496-502. Review
59. Kihara A, Sakuraba H, Ikeda M, Denpoh A, Igarashi Y (2008) Membrane topology and essential amino acid residues of Phs1, a 3-hydoroxyacyl-CoA Dehydratase involved in very long-chain fatty acid elongation. J Biol Chem, 283, 11199-11209.
58. Ikeda M, Kihara A, Denpoh A, Igarashi Y (2008) The Rim101 pathway is involved in Rsb1 expression induced by altered lipid asymmetry. Mol Biol Cell, 19, 1922-1931.
57. Iwaki S, Sano T, Takagi T, Osumi M, Kihara A, Igarashi Y (2007) Intracellular trafficking pathway of yeast long-chain base kinase Lcb4, from its sythesis to its degradation. J Biol Chem, 282, 28485-28492.
56. Kasahara K, Nakayama Y, Kihara A, Matsuda D, Ikeda K, Kuga T, Fukumoto Y, Igarashi Y, Yamaguchi N (2007) Rapid trafficking of c-Src, a non-palmitoylated Src-family kinase, between the plasma membrane and late endosomes/lysosomes. Exp Cell Res, 313, 2651-2666.
55. Anada Y, Igarashi Y, Kihara A (2007) The immunomodulator FTY720 is phosphorylated and released from platelets. Eur J Pharm, 56, 106-111.
54. Kihara A, Mitsutake S, Mizutani Y, Igarashi Y (2007) Metabolism and biological functions of two phosphorylated sphingolipids, sphingosine 1-phosphate and ceramide1-phosphate. Prog Lipid Res, 46, 126-144. Review
53. Ito K, Anada Y, Tani M, Ikeda M, Sano T, Kihara A, Igarashi Y (2007) Lack of sphingosine 1-phosphate (S1P)-degrading enzymes in erythrocytes, a potential major source of plasma S1P in addition to platelets. Biochem Biophys Res Commun, 357, 212-217.
52. 五十嵐靖之，木原章雄 (2007) スフィンゴ脂質の生理活性と代謝調節. バイオとナノの融合 II 新生命科学の応用 (北海道大学出版会), 117-131. Review
51. Uemura S, Kihara A, Iwaki S, Inokuchi JI, Igarashi Y (2007) Regulation of the transport and protein levels of the inositol phosphorylceramide mannosyltransferases Csg1 and Csh1 by the Ca²⁺-binding protein Csg2. J Biol Chem, 282, 8613-8621.
50. Jin YX, Yoo HS, Kihara A, Choi CH, Oh S, Moon DC, Igarashi Y, Lee YM (2006) Sphingosine kinase assay system with fluorescent detection in high performance liquid chromatography. Arch Pharm Res, 29, 1049-1054.
49. 木原章雄 (2006) 多彩な機能を持つスフィンゴ脂質，スフィンゴシン(長鎖塩基)１-リン酸の生理的役割，生成/代謝，細胞内動態. 生化学, 78, 725-737. Review
48. Ikeda M, Kihara A, Igarashi Y (2006) Lipid asymmetry of the eukaryotic plasma membrane: functions and related enzymes.Biol Pharm Bull, 29, 1542-1546. Review
47. Mizutani Y, Kihara A, Igarashi Y (2006) LASS3 (longevity assurance homologue 3) is a mainly testis-specific (dihydro)ceramide synthase with relatively broad substrate specificity. Biochem J, 398, 531-538.
46. Kihara A, Igarashi Y (2006) Synthesis, metabolism, and trans-bilayer movement of long-chain base. Sphingolipid Biology (ed. Y. Hirabayashi, Y. Igarashi, and A. H. Merrill, Jr, Springer-Verlag, Tokyo), 95-106. Review
45. Igarashi Y, Kihara A, Hirabayashi Y (2006) The genes in yeast and mammals. Sphingolipid Biology (ed. Y. Hirabayashi, Y. Igarashi, and A. H. Merrill, Jr, Springer-Verlag, Tokyo), 515-517.
44. Kihara A, Anada Y, Igarashi Y (2006) Mouse sphingosine kinase isoforms SPHK1a and SPHK1b differ in enzymatic traits including stability, localization, modification, and oligomerization. J Biol Chem, 281, 4532-4539.
43. Ohno Y, Kihara A, Sano T, Igarashi Y (2006) Intracellular localization and tissue-specific distribution of human and yeast DHHC cysteine-rich domain-containing proteins. Biochim Biophys Acta, 2761, 474-483.
42. Tani M, Kihara A, Igarashi Y (2006) Rescue of cell growth by sphingosine with disruption of lipid microdomain formation in Saccharomyces cerevisiae deficient in sphingolipid biosynthesis. Biochem J, 394, 237-242.
41. Kobayashi N, Nishi T, Hirata T, Kihara A, Sano T, Igarashi Y, Yamaguchi A (2006) Sphingosine 1-phosphate is released from the cytosol of rat platelets in a carrier-mediated manner. J Lipid Res, 47, 614-621.
40. Hiraga Y, Kihara A, Sano T, Igarashi Y (2006) Changes in S1P₁ and S1P₂ expression during embryonal development and primitive endoderm differentiation of F9 cells. Biochem Biophys Res Commun, 344, 852-858.
39. 木原章雄，岩城壮一郎，五十嵐靖之 (2005) 生理活性脂質スフィンゴシン1－リン酸の機能と代謝. 蛋白質核酸酵素, 50, 1443-1448. Review.
38. Kihara A, Kurotsu F, Sano T, Iwaki S, Igarashi Y (2005) Long-chain base kinase Lcb4 Is anchored to the membrane through its palmitoylation by Akr1. Mol Cell Biol, 25, 9189-9197.
37. Sano T, Kihara A, Kurotsu F, Iwaki S, Igarashi Y (2005) Regulation of the sphingoid long-chain base kinase Lcb4p by ergosterol and heme: studies in phytosphingosine-resistant mutants. J Biol Chem, 280, 36674-36682.
36. Mizutani Y, Kihara A, Igarashi Y (2005) Mammalian Lass6 and its related family members regulate synthesis of specific ceramides. Biochem J, 390, 263-271.
35. Kariya Y, Kihara A, Ikeda M, Kikuchi F, Nakamura S, Hashimoto S, Choi CH, Lee YM, Igarashi Y (2005) Products by the sphingosine kinase/sphingosine 1-phosphate (S1P) lyase pathway but not S1P stimulate mitogenesis. Genes Cells, 10, 605-615.
34. Iwaki S, Kihara A, Sano T, Igarashi Y (2005) Phosphorylation by Pho85 cyclin-dependent kinase acts as a signal for the down-regulation of the yeast sphingoid long-chain base kinase Lcb4 during the stationary phase. J Biol Chem, 280, 6520-6527.
33. Ikeda M, Kihara A, Kariya Y, Lee YM, Igarashi Y (2005) Sphingolipid-to-glycerophospholipid conversion in SPL-null cells implies the existence of an alternative isozyme. Biochem Biophys Res Commun, 329, 474-479.
32. Kihara A, Igarashi Y (2004) Cross talk between sphingolipids and glycerophospholipids in the establishment of plasma membrane asymmetry. Mol Biol Cell, 15, 4949-4959.
31. Kihara A, Igarashi Y (2004) FVT-1 is a mammalian 3-ketodihydrosphingosine reductase with an active site that faces the cytosolic side of the endoplasmic reticulum membrane. J Biol Chem, 279, 49243-49250.
30. Yokota S, Taniguchi Y, Kihara A, Mitsutake S, Igarashi Y (2004) Asp177 in C4 domain of mouse sphingosine kinase 1a is important for the sphingosine recognition. FEBS Lett, 578, 106-110.
29. Mizutani Y, Kihara A, Igarashi Y (2004) Identification of the human sphingolipid C4-hydroxylase, hDES2, and its up-regulation during keratinocyte differentiation. FEBS Lett, 563, 93-97.
28. Ikeda M, Kihara A, Igarashi Y (2004) Sphingosine-1-phosphate lyase SPL is an endoplasmic reticulum-resident, integral membrane protein with the pyridoxal 5'-phosphate binding domain exposed to the cytosol. Biochem Biophys Res Commun, 325, 338-343.
27. Kihara A, Ikeda M, Kariya Y, Lee EY, Lee YM, Igarashi Y (2003) Sphingosine-1-phosphate lyase is involved in the differentiation of F9 embryonal carcinoma cells to primitive endoderm. J Biol Chem, 278, 14578-14585.
26. Kihara A, Sano T, Iwaki S, Igarashi Y (2003) Transmembrane topology of sphingoid long-chain base-1-phosphate phosphatase, Lcb3p. Genes Cells, 8, 525-535.
25. Uemura S, Kihara A, Inokuchi J, Igarashi Y (2003) Csg1p and newly identified Csh1p function in mannosylinositol phosphorylceramide synthesis by interacting with Csg2p. J Biol Chem, 278, 45049-45055.
24. Ogawa C, Kihara A, Gokoh M, Igarashi Y (2003) Identification and characterization of a novel human sphingosine-1-phosphate phosphohydrolase, hSPP2. J Biol Chem, 278, 1268-1272.
23. Fukuda Y, Kihara A, Igarashi Y (2003) Distribution of sphingosine kinase activity in mouse tissues: contribution of SPHK1. Biochem Biophys Res Commun, 309, 155-160.
22. 木原章雄，五十嵐靖之 (2002) 酵母におけるスフィンゴイド塩基１－リン酸の役割と細胞内動態. 蛋白質核酸酵素, 47(4 Suppl), 431-437. Review.
21. 木原章雄 (2002) 酵母セラミド合成酵素の同定とその遺伝子欠損株の解析. ファルマシア, 38, 549. Review.
20. Kihara A, Igarashi Y (2002) Identification and characterization of a Saccharomyces cerevisiae gene, RSB1, involved in sphingoid long-chain base release. J Biol Chem, 277, 30048-30054.
19. Kihara A, Noda T, Ishihara N, Ohsumi Y (2001) Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and carboxypeptidase Y sorting in Saccharomyces cerevisiae. J Cell Biol, 152, 519-530.
18. Kihara A, Kabeya Y, Ohsumi Y, Yoshimori T (2001) Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network. EMBO Rep, 2, 330-335.
17. Kihara A, Akiyama Y, Ito K (2001) Revisiting the lysogenization control of bacteriophage λ. Identification and characterization of a new host component, HflD. J Biol Chem, 276, 13695-13700.
16. Ishihara N, Hamasaki M, Yokota S, Suzuki K, Kamada Y, Kihara A, Yoshimori T, Noda T, Ohsumi Y (2001) Autophagosome requires specific early Sec proteins for its formation and NSF/SNARE for vacuolar fusion. Mol Biol Cell, 12, 3690-3702.
15. Kihara A, Akiyama Y, Ito K (1999) Dislocation of membrane proteins in FtsH-mediated proteolysis. EMBO J, 18, 2970-2981.
14. Kai Y, Matsumura H, Inoue T, Terada K, Nagara Y, Yoshinaga T, Kihara A, Tsumura K, Izui K (1999) Three-dimensional structure of phosphoenolpyruvate carboxylase: a proposed mechanism for allosteric inhibition. Proc Natl Acad Sci USA, 96, 823-828.
13. Kihara A, Ito K (1998) Translocation, folding, and stability of the HflKC complex with signal anchor topogenic sequences. J Biol Chem, 273, 29770-29775.
12. Kihara A, Akiyama Y, Ito K (1998) Different pathways for protein degradation by the FtsH/HflKC membrane-embedded protease complex: an implication from the interference by a mutant form of a new substrate protein, YccA. J Mol Biol, 279, 175-188.
11. Akiyama Y, Kihara A, Mori H, Ogura T, Ito K (1998) Roles of the periplasmic domain of Escherichia coli FtsH (HflB) in protein interactions and activity modulation. J Biol Chem, 273, 22326-33.
10. Akiyama Y, Ehrmann M, Kihara A, Ito K (1998) Polypeptide binding of Escherichia coli FtsH (HflB). Mol Microbiol, 28, 803-812.
9. Kihara A, Akiyama Y, Ito K (1997) Host regulation of lysogenic decision in bacteriophage λ: transmembrane modulation of FtsH (HflB), the cII degrading protease, by HflKC (HflA). Proc Natl Acad Sci USA, 94, 5544-5549.
8. Ito K, Akiyama Y, Kihara A, Matsuo E, Homma T, Shirai Y, Yoshihisa T, Taura T, Shimoike T, Sone M, Matsumoto G (1997) The SecY Complex and the FtsH complex in the E. coli Plasma Membrane. MEMBRANE PROTEINS: Structure, Function and Expression Control (ed. N. Hamasaki and K. Mihara, Kyushu University Press/ Karger Medical and Scientific Publishers, Fukuoka and Basel), 169-181. Review
7. Kihara A, Akiyama Y, Ito K (1996) A protease complex in the Escherichia coli plasma membrane: HflKC (HflA) forms a complex with FtsH (HflB), regulating its proteolytic activity against SecY. EMBO J, 15, 6122-6131.
6. Akiyama Y, Kihara A, Tokuda H, Ito K (1996) FtsH (HflB) is an ATP-dependent protease selectively acting on SecY and some other membrane proteins. J Biol Chem, 271, 31196-31201.
5. Akiyama Y, Kihara A, Ito K (1996) Subunit a of proton ATPase F₀ sector is a substrate of the FtsH protease in Escherichia coli. FEBS Lett, 399, 26-28.
4. Izui K, Terada K, Yano M, Nakamura T, Abe K, Kihara A, Yoshioka I, Takahashi M (1995) Phosphoenolpyruvate carboxylase: alteration of catalytic and regulatory properties by site-directed mutagenesis and isolation of the gene from an extreme thermophile. Energy Convers Mgmt, 36, 751-754.
3. Shimoike T, Taura T, Kihara A, Yoshihisa T, Akiyama Y, Cannon K, Ito K (1995) Product of a new gene, syd, functionally interacts with SecY when overproduced in Escherichia coli. J Biol Chem, 270, 5519-5526.
2. Homma T, Yoshihisa T, Kihara A, Akiyama Y, Ito K (1995) Intracellular stability of α fragments of β-galactosidase: effects of amino-terminally fused polypeptides. Biochem Biophys Res Commun, 215, 452-458.
1. Kihara A, Akiyama Y, Ito K (1995) FtsH is required for proteolytic elimination of uncomplexed forms of SecY, an essential protein translocase subunit. Proc Natl Acad Sci USA, 92, 4532-4536.

修士課程及び博士後期課程大学院生を募集しています。
進学希望の方は下記までご連絡ください。

〒060-0812
札幌市北区北12条西6丁目
北海道大学大学院薬学研究院
生化学研究室
木原　章雄
Tel : 011-706-3754
Fax : 011-706-4900
Email : kihara@pharm.hokudai.ac.jp