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学部担当授業科目

  生物化学実習 I, II,先端生物科学実験法 I

Publication lists of Yusuke Ohno

36. Nohara T, Ohno Y, Kihara A. (2020)
Impaired production of the skin barrier lipid acylceramide by CYP4F22 ichthyosis mutations. J Dermatol Sci, 101, 69-71.

35. Kawana M, Miyamoto M, Ohno Y, Kihara A. (2020)
Comparative profiling and comprehensive quantification of stratum corneum ceramides in humans and mice by LC/MS/MS. J Lipid Res, 61, 884-895.

34. Wang ZN, Kawabori M, Higashikawa K, Yasui H, Kuge Y, Ohno Y, Kihara A, Midori YA, Houkin K. (2020)
FTY720 protects against ischemia-reperfusion injury by preventing the redistribution of tight junction proteins and decreases inflammation in the sub-acute phase in an experimental stroke model. Transl Stroke Res, in press

33. Yamamoto H, Hattori M, Chamulitrat W, Ohno Y, Kihara A. (2020)
Skin permeability barrier formation by the ichthyosis-causative gene FATP4 through formation of the barrier lipid ω-O-acylceramide. Proc Natl Acad Sci USA, 117, 2914-2922.
(プレスリリース:北大AMED

32. Jojima K, Edagawa M, Sawai M, Ohno Y, Kihara A. (2020)
Biosynthesis of the anti-lipid-microdomain sphingoid base 4,14-sphingadiene by the ceramide desaturase FADS3. FASEB J, 34, 3318-3335.

31. 酒井祥太,大野祐介 (2019)
スフィンゴ脂質のリピドミクス概略. セラミド研究の新展開 〜基礎から応用へ〜 (セラミド研究会編,食品化学新聞社), 99-105.

30. Kinugasa Y, Hirano Y, Sawai M, Ohno Y, Shindo T, Asakawa H, Chikashige Y, Shibata S, Kihara A, Haraguchi T, Hiraoka Y. (2019)
Very-long-chain fatty acid elongase Elo2 rescues lethal defects associated with loss of the nuclear barrier function. J Cell Sci, 229021.

29. Ohno Y, Nara A, Nakamichi S, Kihara A. (2018)
Molecular mechanism of the ichthyosis pathology of Chanarin-Dorfman syndrome: stimulation of PNPLA1-catalyzed ω-O-acylceramide production by ABHD5. J Dermatol Sci, 92, 245-253.

28. Swamy M, Murai Y, Ohno Y, Jojima K, Kihara A, Mitsutake S, Igarashi Y, Yu J, Yao M, Suga Y, Anetai M, Monde K. (2018)
Structure-inspired design of a sphingolipid mimic sphingosine-1-phosphate receptor agonist from a naturally occurring sphingomyelin synthase inhibitor. ChemComm, 54, 12758-12761.

27. Edagawa M, Sawai M, Ohno Y, Kihara A. (2018)
Widespread tissue distribution and synthetic pathway of polyunsaturated C24:2 sphingolipids in mammals. Biochim Biophys Acta, 1863, 1441-1448.

26. Honda Y, Kitamura T, Naganuma T, Abe T, Ohno Y, Sassa T, Kihara A. (2017)
Decreased skin barrier lipid acylceramide and differentiation-dependent gene expression in ichthyosis gene Nipal4 knockout mice. J Invest Dermatol, 138, 741-749.

25. Sawai M, Uchida Y, Ohno Y, Miyamoto M, Nishioka C, Itohara S, Sassa T, Kihara A. (2017) The 3-hydroxyacyl-CoA dehydratases HACD1 and HACD2 exhibit functional redundancy and are active in a wide range of fatty acid elongation pathways. J Biol Chem, 292, 15538-15551.

24. Ohno Y. (2017)
Elucidation of the Synthetic Mechanism of Acylceramide, an Essential Lipid for Skin Barrier Function. Yakugaku Zasshi, 137, 1201-1208.

23. Takeichi T, Torrelo A, Lee JYW, Ohno Y, Lozano ML, Kihara A, Liu L, Yasuda Y, Ishikawa J, Murase T, Rodrigo AB, Fernandez-Crehuet P, Toi Y, Mellerio J, Rivera J, Vicente V, Kelsell DP, Nishimura Y, Okuno Y, Kojima D, Ogawa Y, Sugiura K, Simpson MA, McLean WHI, Akiyama M, McGrath JA. (2017)
Biallelic mutations in KDSR disrupt ceramide synthesis and result in a spectrum of keratinization disorders associated with thrombocytopenia. J Invest Dermatol, in press.

22. Ohno Y, Kamiyama N, Nakamichi S, Kihara A. (2017)
Formation of the skin barrier lipid ω-O-acylceramide by the ichthyosis gene PNPLA1. Nat Commun, 8, 14610.

21. Yamamoto S, Yako Y, Fujioka Y, Kajita M, Kameyama T, Kon S, Ishikawa S, Ohba Y, Ohno Y, Kihara A, Fujita Y. (2016)
A role of the sphingosine-1-phosphate (S1P)- S1P receptor 2 pathway in epithelial defense against cancer (EDAC). Mol Biol Cell, 27, 491-499.

20. Ohno Y, Nakamichi S, Ohkuni A, Kamiyama N, Naoe A, Tsujimura H, Yokose U, Sugiura K, Ishikawa J, Akiyama M, Kihara A (2015)
Essential role of the cytochrome P450 CYP4F22 in the production of acylceramide, the key lipid for skin permeability barrier formation.
Proc Natl Acad Sci USA
, 112, 7707-7712. (プレスリリースの内容はこちら

19. Blondelle J*, Ohno Y*, Gache V, Guyot S, Storck S, Blanchard-Gutton N, Barthélémy I, Walmsley G, Rahier A, Gadin S, Maurer M, Guillaud L, Prola A, Ferry A, Aubin-Houzelstein G, Demarquoy J, Relaix F, Piercy R J, Blot S, Kihara A, Tiret L, Pilot-Storck F (2015)
*equally contributed
HACD1, a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth.
J Mol Cell Biol, 7, 429-440.

18. Kondo N, Ohno Y, Yamagata M, Obara T, Seki N, Kitamura T, Naganuma T, Kihara A (2014) Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acids. Nat Commun, 5, 5338.

17. Sassa T, Wakashima T, Ohno Y, Kihara A (2014)
Lorenzo's oil inhibits ELOVL1 and lowers the level of sphingomyelin with a saturated very long-chain fatty acid.
J Lipid Res, 55, 524-530.
ASBMB のブログ「WILD TYPES」で紹介

16. Abe K, Ohno Y, Sassa T, Taguchi R, Caliskan M, Ober C, Kihara A (2013)
Mutation for non-syndromic mental retardation in the trans-2-enoyl-CoA reductase TER gene involved in fatty acid elongation impairs the enzyme activity and stability leading to change in sphingolipid profile.
J Biol Chem
, 288, 36741-36749.

15. Ohkuni A, Ohno Y, Kihara A (2013)
Identification of acyl-CoA synthetases involved in the mammalian sphingosine 1-phosphate metabolic pathway.
Biochem Biophys Res Commun
, 442, 195-201.

14. Muhammad E, Reish O, Ohno Y, Scheetz T, DeLuca A, Searby C, Regev M, Benyamini L, Fellig Y, Kihara A, Sheffield VC, Parvari R (2013)
Congenital myopathy is caused by mutation of HACD1. Hum Mol Genet, 22, 5229-5236.

13 . Kitamura T, Naganuma T, Abe K, Nakahara K, Ohno Y, Kihara A (2013)
Substrate specificity, plasma membrane localization, and lipid modification of the aldehyde dehydrogenase ALDH3B1.
Biochim. Biophys. Acta, 1831, 1394-1401.

12. Mizutani Y, Sun H, Ohno Y, Sassa T, Wakashima T, Obara M, Yuyama K, Kihara A, Igarashi Y (2013)
Cooperative synthesis of ultra long-chain fatty acid and ceramide during keratinocyte differentiation.
PLoS One
, 8, e67317.

11. Sassa T, Ohno Y, Suzuki S, Nomura T, Nishioka C, Kashiwagi T, Hirayama T, Akiyama M, Taguchi R, Shimizu H, Itohara S, Kihara A (2013)
Impaired epidermal permeability barrier in mice lacking the Elovl1 gene responsible for very long-chain fatty acid production.
Mol. Cell Biol., 33, 2787-2796.

10. Ohno Y, Kashio A, Ogata R, Ishitomi A, Yamazaki Y, Kihara A (2012)
Analysis of substrate specificity of human DHHC protein acyltransferases using a yeast expression system.
Mol. Biol. Cell
, 23, 4543-4551.

9. Nakahara K, Ohkuni A, Kitamura T, Abe K, Naganuma T, Ohno Y, Zoeller RA, Kihara A (2012)
Sjogren-Larsson syndrome gene encodes a hexadecenal dehydrogenase of the sphingosine 1-phosphate degradation pathway.
Mol. Cell, 41, 461-471.

8. 大野祐介,木原章雄 (2011)
極長鎖脂肪酸伸長とセラミド合成
ここまできたセラミド研究最前線 セラミド -基礎と応用-(セラミド研究会編,食品化学新聞社)
43-49.

7. Naganuma T.,Sato Y., Sassa T., Ohno Y., Kihara A. (2011)
Biochemical characterization of the very long-chain fatty acid elongase ELOVL7.
FEBS Lett., 585, 3337-3341.

6. Okuda, A., Naganuma, T., Ohno, Y., Abe, K., Yamagata, M., Igarashi, Y., and Kihara, A. (2010)
Hetero-oligomeric interactions of an ELOVL4 mutant protein: implications in the molecular mechanism of Stargardt-3 macular dystrophy.
Mol. Vision, 16, 2438-2445.

5. Ohno, Y., Suto, S., Yamanaka, M., Mizutani, Y., Mitsutake, S., Igarashi, Y., Sassa, T., and Kihara, A. (2010)
ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis.
Proc. Natl. Acad. Sci. USA, 107, 18439-18444.

4. Konishi, H., Okuda, A., Ohno, Y., and Kihara, A. (2010)
Characterization of HACD1 K64Q mutant found in arrhythmogenic right ventricular dysplasia patients.
J. Biochem., 148, 617-622.

3. Ohno, Y., Ito, A., Ogata, R., Hiraga, Y., Igarashi, Y., and Kihara, A. (2009)
Palmitoylation of the sphingosine 1-phosphate receptor S1P1 is involved in its signaling functions and internalization.
Genes Cells, 14, 911-923.

2. Balan, L., Foltyn, V. N., Zehl, M., Dumin, E., Dikopoltsev, E., Knoh, D., Ohno, Y., Kihara, A., Jensen, O. N., Radzishevsky, I. S., and Wolosker, H. (2009)
Feedback inactivation of D-serine synthesis by NMDA receptor-elicited translocation of serine racemase to the membrane.
Proc. Natl. Acad. Sci. U. S. A.,106, 7589-7594.

1. Ohno Y, Kihara A, Sano T, Igarashi Y. (2006)
Intracellular localization and tissue-specific distribution of human and yeast DHHC cysteine-rich domain-containing proteins.
Biochim. Biophys. Acta. 2761, 474-483.

 

修士課程及び博士後期課程大学院生を募集しています。
進学希望の方は下記までご連絡ください。

〒060-0812
札幌市北区北12条西6丁目
北海道大学大学院薬学研究院
生化学研究室
木原 章雄
Tel : 011-706-3754
Fax : 011-706-4900
Email : kihara@pharm.hokudai.ac.jp