Achievements

Reviewed Papers

  1. Hibino M, Filosi T, Carrion LL, Porcu E, Malhota A, Lüdtke-Buzug K, Ibrahim SM, Yamada Y#, Hirose M#An efficient approach to modulate mitochondrial function in murine primary macrophages by mitochondria-targeting nanocapsule, MITO-Porter. Biomedicine & Pharmacotherapy 186: 118019 (2025) DOI: 10.1016/j.biopha.2025.118019
  2. Endo R, Ueda T, Nagaoki T, Sato Y, Maishi N, Hida K, Harashima H*, Nakamura T*.Selective vascular disrupting therapy by lipid nanoparticle-mediated Fas ligand silencing and stimulation of STING. Biomaterials 321: 123297 (2025). DOI: 10.1016/j.biomaterials.2025.123297
  3. Suzuki Y, Yakuwa M, Sato M, Samaridou E, Beck-Broichsitter M, Maeki M, Tokeshi M, Yamada Y, Harashima H, Sato Y.#Splenic B cell-targeting lipid nanoparticles for safe and effective mRNA vaccine delivery.J Control Release. 2025 Apr 3;382:113687. DOI: 10.1016/j.jconrel.2025.113687
  4. Iwakawa K, Sato R, Konaka M, Yamada Y, Harashima H, Sato Y.Adv Sci (Weinh). # Cubic Phase-Inducible Zwitterionic Phospholipids Improve the Functional Delivery of mRNA. 2025 Feb 17:e2413016. DOI: 10.1002/advs.202413016
  5. Zhao H, Naganawa R, Yamada Y, Osakada Y, Fujitsuka M, Mitomo H, Miyatake Y, Harashima H, Biju V, Takano Y, π-extended porphyrin-based near-infrared photosensitizers for mitochondria-targeted photodynamic therapy. J. Photochem. Photobiol. A Chem., 449: 115397 (2024) DOI: 10.1016/j.jphotochem.2023.115397.
  6. Shiraishi M, Sasaki D, Hibino M, Takeda A, Harashima H, Yamada Y#, Human cardiosphere-derived cells with activated mitochondria for better myocardial regenerative therapy. J. Control. Release., 367: 486-499 (2024) DOI: 10.1016/j.jconrel.2024.01.058.
  7. Sato I, Hibino M, Takeda A, Harashima H, Yamada Y, Activation of mitochondrial oxygen consumption rate by delivering coenzyme Q10 to mitochondria of rat skeletal muscle cell (L6). J. Pharm. Sci. 113 (7): 1836-1843 (2024). DOI: 10.1016/j.xphs.2024.01.020
  8. Nakamura T, Sasaki S, Sato Y, Harashima H. Cancer immunotherapy with lipid nanoparticles loaded with a STING agonist against renal tumor lung metastasis. Pharmaceutics 16: 31 (2024) DOI: 10.3390/pharmaceutics16010031
  9. Naganawa R, Zhao H, Takano Y, Maeki M, Tokeshi M, Harashima H, Yamada Y. Investigation of the nanoparticulation method and cell-killing effect following the mitochondrial delivery of hydrophobic porphyrin-based photosensitizers.International Journal of Molecular Sciences 25: 4294 (2024). DOI: 10.3390/ijms25084294.
  10. Khalifa AM, Nakamura T, Sato Y, Harashima H. Vaccination with a combination of STING agonist-loaded lipid nanoparticles and CpG-ODNs protects against lung metastasis via the induction of CD11bhighCD27low memory-like NK cells. Exp Hematol Oncol 13: 36 (2024). DOI: 10.1186/s40164-024-00502-w
  11. Endo R, Ueda T, Nagaoki T, Shima N, Sato Y, Harashima H*, Nakamura T. Impact of in vivo fate of STING agonist-loaded lipid nanoparticles on antitumor immunity. J Control Release 372: 609-618 (2024). DOI: 10.1016/j.jconrel.2024.06.064
  12. Leung J, Strong C, Badior K, Robertson M, Wu X, Meledeo MA, Kang E, Paul M, Sato Y, Harashima H, Cap AP, Devine DV, Jan E, Cullis PR, Kastrup CJ, Genetically engineered transfusable platelets using mRNA lipid nanoparticles, Sci Adv, 9(48): eadi0508 DOI: 10.1126/sciadv.adi0508
  13. Hashiba K, Taguchi M, Sakamoto S, Otsu A, Maeda Y, Ebe H, Okazaki A, Harashima H, Sato Y, Overcoming Thermostability Challenges in mRNA–Lipid Nanoparticle Systems with Piperidine-Based Ionizable Lipids, Commun Biol, 7: 556 DOI: 10.1038/s42003-024-06235-0
  14. Onuma H, Shimizu R, Suzuki Y, Sato M, Harashima H, Sato Y, Engineering Branched Ionizable Lipid for Hepatic Delivery of CRISPR/Cas9 Ribonucleoproteins, iScience (in press)
  15. Maruo Y, Shiraishi M, Hibino M, Abe J, Takeda A, Yamada Y#, Activation of Mitochondria in Mesenchymal Stem Cells by Mitochondrial Delivery of Coenzyme Q10. Biol. Pharm. Bull. 47: 1415-1421 (2024). DOI: 10.1248/bpb.b24-00284
  16. Hori I, Harashima H, Yamada Y#, Different effects of berberine delivery to mitochondria on cells derived from the neural crest. Biol. Pharm. Bull. 47: 1530-1537 (2024) DOI: 10.1248/bpb.b24-00456
  17. Kubota F, Satrialdi, Takano Y, Maeki M, Tokeshi M, Harashima H, Yamada Y, Fine-tuning the encapsulation of a photosensitizer in nanoparticles reveals the relationship between internal structure and phototherapeutic effects. J. Biophotonics. 16(3): e202200119 (2022). DOI: 10.1002/jbio.202200119.
  18. Hori I, Harashima H, Yamada Y, Development of a mitochondrial targeting lipid nanoparticle encapsulating berberine. Int. J. Mol. Sci. 24(2): 903 (2023). DOI: 10.3390/ijms24020903.
  19. Yamaguchi A, Mukai Y, Sakuma T, Narumi K, Furugen A, Yamada Y, Kobayashi M., Monocarboxylate transporter 4 involves in energy metabolism and drug sensitivity in hypoxia. Sci Rep. 13(1): 1501 (2023). DOI: 10.1038/s41598-023-28558-4.
  20. Hibino M, Maeki M, Tokeshi M, Ishitsuka Y, Harashima H, Yamada Y, A System that Delivers an Antioxidant to Mitochondria for the treatment of Drug-Induced Liver Injury. Sci. Rep. 13(1): 6961 (2023). DOI: 10.1038/s41598-023-33893-7.
  21. Tsuneishi T, Kojima K, Kubota F, Harashima H, Yamada Y, Sudo Y, Development of light-induced disruptive liposomes (LiDL) as a photoswitchable carrier for intracellular substance delivery. Chem. Commun. 59(49): 7591-7594 (2023). DOI: 10.1039/d3cc02056h.
  22. Yamada Y, Daikuhara S, Tamura A, Nishida K, Yui N, Harashima H, Differences in the Intracellular Localization of Methylated β-Cyclodextrins-Threaded Polyrotaxanes Lead to Different Cellular States. Biomolecules. 13 (6): 903 (2023). DOI: 10.3390/biom13060903.
  23. Younis MA, Sato Y, Elewa YHA, Kon Y, Harashima H, Reprogramming activated hepatic stellate cells by siRNA-loaded nanocarriers reverses liver fibrosis in mice, J Control Release, 361: 592-603, 2023. DOI: 10.1016/j.jconrel.2023.08.021
  24. Elwakil M, Suzuki R, Khalifa AM, Elshami RM, Isono T, Elewa Y, Sato Y, Nakamura T, Satoh T, Harashima H, Harnessing Topology and Stereochemistry of Glycidylamine-Derived Lipid Nanoparticles for in vivo mRNA Delivery to Immune cells and their Application for Cancer Vaccination, Adv Funct Mater, 2023 in press. DOI: 10.1002/adfm.202303795
  25. Nakamura T, Nakade T, Sato Y, Harashima H, Delivering mRNA to a human NK cell line, NK-92 cells, by lipid nanoparticles, Int J Pharm, 636: 122810, 2023 DOI: 10.1016/j.ijpharm.2023.122810
  26. Kafetzis KN, Papalamprou N, McNulty E, Thong KX, Sato Y, Mironov A, Purohit A, Welsby P, Harashima H, Yu-Wai-Man C, Taga-lakis AD, The effect of cryoprotectants and storage conditions on the transfection efficiency, stability and safety of lipid-based nanoparticles for mRNA and DNA delivery, Adv Healthcare Mater, 2023 DOI: 10.1002/adhm.202203022
  27. Onuma H, Sato Y, Harashima H, Lipid nanoparticle-based ribonucleoprotein delivery for in vivo genome editing, J Control Release, 255: 406-416, 2023 DOI: 10.1016/j.jconrel.2023.02.008
  28. Maeki M, Okada Y, Uno S, Sugiura K, Suzuki Y, Okuda K, Sato Y, Ando M, Yamazaki H, Takeuchi M, Ishida A, Tani H, Harashima H, Tokeshi M, Mass production system for RNA-loaded lipid nanoparticles using piling up microfluidic devices, Appl Mater Today, 31: 101754, 2023 DOI: 10.1016/j.apmt.2023.101754
  29. Younis MA, Sato Y, Elewa YHA, Kon Y, Harashima H, Self-homing nanocarriers for mRNA delivery to the activated hepatic stellate cells in liver fibrosis, J Control Release, 353: 685-698, 2023. DOI: 10.1016/j.jconrel.2022.12.020
  30. Hori I, Harashima H, Yamada Y, Development of liposomes that target axon terminals encapsu-lating berberine in cultured primary neurons. Pharmaceutics., 16(1): 49 (2023) DOI: 10.3390/pharmaceutics16010049.

Press Releases

  • 2023年8月15日　「Professor Yuma Yamada will showcase the MITO-Porter at Targeting Mitochondria 2023」（EurekAlert!） Link

International Presentations

  1. Sato Y, Onuma H, Shimizu R, Harashima H. ”LIPID NANOPARTICLES FOR DELIVERY OF CRISPR/CAS9 RIBONUCLEOPROTEINS.” 18th Liposome Research Days, Jun 26-29, 2024, Glasgow, United Kingdom.
  2. Younis MA, Sato Y, Harashima H. ”RNA-LOADED LIPID NANOPARTICLES REPROGRAM ACTIVATED HEPATIC STELLATE CELLS AND REVERSE LIVER FIBROSIS IN VIVO.” 18th Liposome Research Days, Jun 26-29, 2024, Glasgow, United Kingdom.
  3. Suzuki Y, Sato Y, Harashima H. ”DEVELOPMENT OF SPLENIC B CELL TARGETED mRNA DELIVELY SYSTEM FOR CANCER VACCINE.” 18th Liposome Research Days, Jun 26-29, 2024, Glasgow, United Kingdom.
  4. Sato I, Yamada Y, Hibino M, Sasaki D, Takeda A, Harashima H, Skeletal muscle mitochondria-targeted DDS and activation of mitochondrial function. International Symposium, The 143 rd Annual Meeting of the Pharmaceutical Socoiety of Japan (March. 25-28, 2023, Hokkaido Univ., Sapporo, Japan)
  5. Endo R, Nakamura T, Sato Y, Harashima H. Development of a novel nano cancer therapy that induces the destruction of intratumoral vascular. International Symposium, The 143 rd Annual Meeting of the Pharmaceutical Socoiety of Japan (March. 25-28, 2023, Hokkaido Univ., Sapporo, Japan)
  6. Shiraishi M, Sasaki D, Takeda A, Hibino M, Harashima H, Yamada Y, Human cardiosphere-derived cells with activated mitochondria for better myocardial regenerative therapy. 56th Annual Meeting of the Association for European Pediatric and Congenital Cardiology (Apr. 26-29, 2023, The Convention Centre Dublin, Dublin, Ireland)
  7. Yamada Y, Ito M, Hibino M, Sasaki D, Harashima H, Innovative technology for regulating mitochondrial function in host cells. EUROMIT 2023 (Jun. 11-15, 2023, Bologna Congress Center, Bologna, Italy)
  8. Shiraishi M, Sasaki D, Takeda A, Hibino M, Harashima H, Yamada Y, Myocardial regeneration therapy using human cardiosphere-derived cells with activated mitochondria. EUROMIT 2023 (Jun. 11-15, 2023, Bologna Congress Center, Bologna, Italy)
  9. Hibino M, Maeki M, Tokeshi M, Harashima H, Yamada Y, The use of a coenzyme Q10 encapsulated mitochondrial targeting lipid nanoparticle formulation has therapeutic effects on a drug-induced liver injury. EUROMIT 2023 (Jun. 11-15, 2023, Bologna Congress Center, Bologna, Italy)
  10. Sato I, Hibino M, Sasaki D, Takeda A, Harashima H, Yamada Y, Validation of drug delivery and functional activation to mitochondria in skeletal muscle cell. EUROMIT 2023 (Jun. 11-15, 2023, Bologna Congress Center, Bologna, Italy)
  11. Hibino M, Maeki M, Tokeshi M, Harashima H, Yamada Y, Mitochondria-Targeted CoQ10 Therapy for Acetaminophen Liver Injury. 14th edition of the World Congress on Targeting Mitochondria 2023 (Oct. 11-13, 2023, Steigenberger Hotel Am Kanzleramt, Berlin, Germany)
  12. Khalifa AM, Nakamura T, Sato Y, Harashima H. STING agonist loaded lipid nanoparticle and CpG-ODN induces CD11bhigh CD27 low memory-like NK cells that prevent melanoma lung metastasis. SITC 38th Annual Meeting (San Diego Convention Center, San Diego, Nov 1-5, 2023)