Achievements

Reviewed Papers

  1. Hibino M, Filosi T, Carrion LL, Porcu E, Malhota A, Lüdtke-Buzug K, Ibrahim SM, Yamada Y#, Hirose M#An efficient approach to modulate mitochondrial function in murine primary macrophages by mitochondria-targeting nanocapsule, MITO-Porter. Biomedicine & Pharmacotherapy 186: 118019 (2025) DOI: 10.1016/j.biopha.2025.118019
  2. Endo R, Ueda T, Nagaoki T, Sato Y, Maishi N, Hida K, Harashima H*, Nakamura T*.Selective vascular disrupting therapy by lipid nanoparticle-mediated Fas ligand silencing and stimulation of STING. Biomaterials 321: 123297 (2025). DOI: 10.1016/j.biomaterials.2025.123297
  3. Suzuki Y, Yakuwa M, Sato M, Samaridou E, Beck-Broichsitter M, Maeki M, Tokeshi M, Yamada Y, Harashima H, Sato Y.#Splenic B cell-targeting lipid nanoparticles for safe and effective mRNA vaccine delivery.J Control Release. 2025 Apr 3;382:113687. DOI: 10.1016/j.jconrel.2025.113687
  4. Iwakawa K, Sato R, Konaka M, Yamada Y, Harashima H, Sato Y.Adv Sci (Weinh). # Cubic Phase-Inducible Zwitterionic Phospholipids Improve the Functional Delivery of mRNA. 2025 Feb 17:e2413016. DOI: 10.1002/advs.202413016
  5. Sato I, Sasaki D, Abe J, Yamanouchi K, Takeda A, Yamada Y　Improvement of mitochondrial function in Duchenne muscular dystrophy skeletal muscle cells by coenzyme Q10 delivery to mitochondria.　Biochem Biophys Res Commun. 771: 152003 (2025) DOI: 10.1016/j.bbrc.2025.152003
  6. Norota K, Ishizuka S, Hirose M, Sato Y, Maeki M, Tokeshi M, Ibrahim SM, Harashima H, Yamada Y, Lipid nanoparticle delivery of the CRISPR/Cas9 system directly into the mitochondria of cells carrying m.7778G>T mutation in mtDNA (mt-Atp8). Sci. Rep. 15: 18717 (2025) https://www.hokudai.ac.jp/news/2025/06/post-1929.html%20%20https://www.jst.go.jp/pr/announce/20250619/index.html DOI: 10.1038/s41598-025-03671-8
  7. Sato I, Sasaki D, Abe J, Yamanouchi K, Takeda A, Yamada Y☆, Mitochondrial coenzyme Q10 delivery improves energy production in rat cardiac myoblasts and Duchenne muscular dystrophy model rat cardiomyocytes. Biol. Pharm. Bull. (in press)
  8. Suzuki Y, Yakuwa M, Sato M, Samaridou E, Beck-Broichsitter M, Maeki M, Tokeshi M, Yamada Y, Harashima H, Sato Y. Marginal-zone B cells as promising targets of an mRNA-loaded, lipid-nanoparticle cancer vaccine. Next Nanotechnol, 8: 100154 (2025). DOI: 10.1016/j.nxnano.2025.100154
  9. Maeki M, Niwa A, Oyama S, Aratani K, Ito R, Suzuki Y, Sato Y, Ishida A, Harashima H, Tokeshi M, Microfluidic Production of Exosome-Mimicking Lipid Nanoparticles for Enhanced RNA Delivery: Role of Exosomal Proteins, ACS Appl Mater Interfaces, 7(29): 41666-41679 (2025).
  10. Sato M, Samaridou E, Beck-Broichsitter M, Maeki M, Kita S, Tokeshi M, Maenaka K, Harashima H, Sato Y. Examining the impact of storage conditions on the stability of a liquid formulation of mRNA-loaded lipid nanoparticle. Pharmaceutics, 17(9): 1194 (2025).
  11. Zhao H, Naganawa R, Yamada Y, Osakada Y, Fujitsuka M, Mitomo H, Miyatake Y, Harashima H, Biju V, Takano Y, π-extended porphyrin-based near-infrared photosensitizers for mitochondria-targeted photodynamic therapy. J. Photochem. Photobiol. A Chem., 449: 115397 (2024) DOI: 10.1016/j.jphotochem.2023.115397
  12. Shiraishi M, Sasaki D, Hibino M, Takeda A, Harashima H, Yamada Y#, Human cardiosphere-derived cells with activated mitochondria for better myocardial regenerative therapy. J. Control. Release., 367: 486-499 (2024) DOI: 10.1016/j.jconrel.2024.01.058
  13. Sato I, Hibino M, Takeda A, Harashima H, Yamada Y, Activation of mitochondrial oxygen consumption rate by delivering coenzyme Q10 to mitochondria of rat skeletal muscle cell (L6). J. Pharm. Sci. 113 (7): 1836-1843 (2024). DOI: 10.1016/j.xphs.2024.01.020
  14. Nakamura T, Sasaki S, Sato Y, Harashima H. Cancer immunotherapy with lipid nanoparticles loaded with a STING agonist against renal tumor lung metastasis. Pharmaceutics 16: 31 (2024) DOI: 10.3390/pharmaceutics16010031
  15. Naganawa R, Zhao H, Takano Y, Maeki M, Tokeshi M, Harashima H, Yamada Y. Investigation of the nanoparticulation method and cell-killing effect following the mitochondrial delivery of hydrophobic porphyrin-based photosensitizers.International Journal of Molecular Sciences 25: 4294 (2024). DOI: 10.3390/ijms25084294
  16. Khalifa AM, Nakamura T, Sato Y, Harashima H. Vaccination with a combination of STING agonist-loaded lipid nanoparticles and CpG-ODNs protects against lung metastasis via the induction of CD11bhighCD27low memory-like NK cells. Exp Hematol Oncol 13: 36 (2024). DOI: 10.1186/s40164-024-00502-w
  17. Endo R, Ueda T, Nagaoki T, Shima N, Sato Y, Harashima H*, Nakamura T. Impact of in vivo fate of STING agonist-loaded lipid nanoparticles on antitumor immunity. J Control Release 372: 609-618 (2024). DOI: 10.1016/j.jconrel.2024.06.064
  18. Leung J, Strong C, Badior K, Robertson M, Wu X, Meledeo MA, Kang E, Paul M, Sato Y, Harashima H, Cap AP, Devine DV, Jan E, Cullis PR, Kastrup CJ, Genetically engineered transfusable platelets using mRNA lipid nanoparticles, Sci Adv, 9(48): eadi0508 DOI: 10.1126/sciadv.adi0508
  19. Hashiba K, Taguchi M, Sakamoto S, Otsu A, Maeda Y, Ebe H, Okazaki A, Harashima H, Sato Y, Overcoming Thermostability Challenges in mRNA–Lipid Nanoparticle Systems with Piperidine-Based Ionizable Lipids, Commun Biol, 7: 556 DOI: 10.1038/s42003-024-06235-0
  20. Onuma H, Shimizu R, Suzuki Y, Sato M, Harashima H, Sato Y, Engineering Branched Ionizable Lipid for Hepatic Delivery of CRISPR/Cas9 Ribonucleoproteins, iScience (in press)
  21. Maruo Y, Shiraishi M, Hibino M, Abe J, Takeda A, Yamada Y#, Activation of Mitochondria in Mesenchymal Stem Cells by Mitochondrial Delivery of Coenzyme Q10. Biol. Pharm. Bull. 47: 1415-1421 (2024). DOI: 10.1248/bpb.b24-00284
  22. Hori I, Harashima H, Yamada Y#, Different effects of berberine delivery to mitochondria on cells derived from the neural crest. Biol. Pharm. Bull. 47: 1530-1537 (2024) DOI: 10.1248/bpb.b24-00456
  23. Kubota F, Satrialdi, Takano Y, Maeki M, Tokeshi M, Harashima H, Yamada Y, Fine-tuning the encapsulation of a photosensitizer in nanoparticles reveals the relationship between internal structure and phototherapeutic effects. J. Biophotonics. 16(3): e202200119 (2022). DOI: 10.1002/jbio.202200119
  24. Hori I, Harashima H, Yamada Y, Development of a mitochondrial targeting lipid nanoparticle encapsulating berberine. Int. J. Mol. Sci. 24(2): 903 (2023). DOI: 10.3390/ijms24020903
  25. Yamaguchi A, Mukai Y, Sakuma T, Narumi K, Furugen A, Yamada Y, Kobayashi M., Monocarboxylate transporter 4 involves in energy metabolism and drug sensitivity in hypoxia. Sci Rep. 13(1): 1501 (2023). DOI: 10.1038/s41598-023-28558-4
  26. Hibino M, Maeki M, Tokeshi M, Ishitsuka Y, Harashima H, Yamada Y, A System that Delivers an Antioxidant to Mitochondria for the treatment of Drug-Induced Liver Injury. Sci. Rep. 13(1): 6961 (2023). DOI: 10.1038/s41598-023-33893-7
  27. Tsuneishi T, Kojima K, Kubota F, Harashima H, Yamada Y, Sudo Y, Development of light-induced disruptive liposomes (LiDL) as a photoswitchable carrier for intracellular substance delivery. Chem. Commun. 59(49): 7591-7594 (2023). DOI: 10.1039/d3cc02056h
  28. Yamada Y, Daikuhara S, Tamura A, Nishida K, Yui N, Harashima H, Differences in the Intracellular Localization of Methylated β-Cyclodextrins-Threaded Polyrotaxanes Lead to Different Cellular States. Biomolecules. 13 (6): 903 (2023). DOI: 10.3390/biom13060903
  29. Younis MA, Sato Y, Elewa YHA, Kon Y, Harashima H, Reprogramming activated hepatic stellate cells by siRNA-loaded nanocarriers reverses liver fibrosis in mice, J Control Release, 361: 592-603, 2023. DOI: 10.1016/j.jconrel.2023.08.021
  30. Elwakil M, Suzuki R, Khalifa AM, Elshami RM, Isono T, Elewa Y, Sato Y, Nakamura T, Satoh T, Harashima H, Harnessing Topology and Stereochemistry of Glycidylamine-Derived Lipid Nanoparticles for in vivo mRNA Delivery to Immune cells and their Application for Cancer Vaccination, Adv Funct Mater, 2023 in press. DOI: 10.1002/adfm.202303795
  31. Nakamura T, Nakade T, Sato Y, Harashima H, Delivering mRNA to a human NK cell line, NK-92 cells, by lipid nanoparticles, Int J Pharm, 636: 122810, 2023 DOI: 10.1016/j.ijpharm.2023.122810
  32. Kafetzis KN, Papalamprou N, McNulty E, Thong KX, Sato Y, Mironov A, Purohit A, Welsby P, Harashima H, Yu-Wai-Man C, Taga-lakis AD, The effect of cryoprotectants and storage conditions on the transfection efficiency, stability and safety of lipid-based nanoparticles for mRNA and DNA delivery, Adv Healthcare Mater, 2023 DOI: 10.1002/adhm.202203022
  33. Onuma H, Sato Y, Harashima H, Lipid nanoparticle-based ribonucleoprotein delivery for in vivo genome editing, J Control Release, 255: 406-416, 2023 DOI: 10.1016/j.jconrel.2023.02.008
  34. Maeki M, Okada Y, Uno S, Sugiura K, Suzuki Y, Okuda K, Sato Y, Ando M, Yamazaki H, Takeuchi M, Ishida A, Tani H, Harashima H, Tokeshi M, Mass production system for RNA-loaded lipid nanoparticles using piling up microfluidic devices, Appl Mater Today, 31: 101754, 2023 DOI: 10.1016/j.apmt.2023.101754
  35. Younis MA, Sato Y, Elewa YHA, Kon Y, Harashima H, Self-homing nanocarriers for mRNA delivery to the activated hepatic stellate cells in liver fibrosis, J Control Release, 353: 685-698, 2023. DOI: 10.1016/j.jconrel.2022.12.020
  36. Hori I, Harashima H, Yamada Y, Development of liposomes that target axon terminals encapsu-lating berberine in cultured primary neurons. Pharmaceutics., 16(1): 49 (2023) DOI: 10.3390/pharmaceutics16010049

Press Releases

  • 2023年8月15日　「Professor Yuma Yamada will showcase the MITO-Porter at Targeting Mitochondria 2023」（EurekAlert!） Link

International Presentations

  1. Takumi Nagaoki, Rikito Endo, Tomoki Ueda, Natsumi Shima, Yusuke Sato, Hideyoshi Harashima, Takashi Nakamura."Effect of characteristics of STING agonist-loaded lipid nanoparticles on cancer immune responses"The 10th Japan-Taiwan Joint Symposium For Pharmaceutical Sciences (Sep 5, 2025, Hokkaido Univ., Sapporo, Japan)
  2. Sato Y, Onuma H, Shimizu R, Harashima H. ”LIPID NANOPARTICLES FOR DELIVERY OF CRISPR/CAS9 RIBONUCLEOPROTEINS.” 18th Liposome Research Days, Jun 26-29, 2024, Glasgow, United Kingdom.
  3. Younis MA, Sato Y, Harashima H. ”RNA-LOADED LIPID NANOPARTICLES REPROGRAM ACTIVATED HEPATIC STELLATE CELLS AND REVERSE LIVER FIBROSIS IN VIVO.” 18th Liposome Research Days, Jun 26-29, 2024, Glasgow, United Kingdom.
  4. Suzuki Y, Sato Y, Harashima H. ”DEVELOPMENT OF SPLENIC B CELL TARGETED mRNA DELIVELY SYSTEM FOR CANCER VACCINE.” 18th Liposome Research Days, Jun 26-29, 2024, Glasgow, United Kingdom.
  5. Sato I, Yamada Y, Hibino M, Sasaki D, Takeda A, Harashima H, Skeletal muscle mitochondria-targeted DDS and activation of mitochondrial function. International Symposium, The 143 rd Annual Meeting of the Pharmaceutical Socoiety of Japan (March. 25-28, 2023, Hokkaido Univ., Sapporo, Japan)
  6. Endo R, Nakamura T, Sato Y, Harashima H. Development of a novel nano cancer therapy that induces the destruction of intratumoral vascular. International Symposium, The 143 rd Annual Meeting of the Pharmaceutical Socoiety of Japan (March. 25-28, 2023, Hokkaido Univ., Sapporo, Japan)
  7. Shiraishi M, Sasaki D, Takeda A, Hibino M, Harashima H, Yamada Y, Human cardiosphere-derived cells with activated mitochondria for better myocardial regenerative therapy. 56th Annual Meeting of the Association for European Pediatric and Congenital Cardiology (Apr. 26-29, 2023, The Convention Centre Dublin, Dublin, Ireland)
  8. Yamada Y, Ito M, Hibino M, Sasaki D, Harashima H, Innovative technology for regulating mitochondrial function in host cells. EUROMIT 2023 (Jun. 11-15, 2023, Bologna Congress Center, Bologna, Italy)
  9. Shiraishi M, Sasaki D, Takeda A, Hibino M, Harashima H, Yamada Y, Myocardial regeneration therapy using human cardiosphere-derived cells with activated mitochondria. EUROMIT 2023 (Jun. 11-15, 2023, Bologna Congress Center, Bologna, Italy)
  10. Hibino M, Maeki M, Tokeshi M, Harashima H, Yamada Y, The use of a coenzyme Q10 encapsulated mitochondrial targeting lipid nanoparticle formulation has therapeutic effects on a drug-induced liver injury. EUROMIT 2023 (Jun. 11-15, 2023, Bologna Congress Center, Bologna, Italy)
  11. Sato I, Hibino M, Sasaki D, Takeda A, Harashima H, Yamada Y, Validation of drug delivery and functional activation to mitochondria in skeletal muscle cell. EUROMIT 2023 (Jun. 11-15, 2023, Bologna Congress Center, Bologna, Italy)
  12. Hibino M, Maeki M, Tokeshi M, Harashima H, Yamada Y, Mitochondria-Targeted CoQ10 Therapy for Acetaminophen Liver Injury. 14th edition of the World Congress on Targeting Mitochondria 2023 (Oct. 11-13, 2023, Steigenberger Hotel Am Kanzleramt, Berlin, Germany)
  13. Khalifa AM, Nakamura T, Sato Y, Harashima H. STING agonist loaded lipid nanoparticle and CpG-ODN induces CD11bhigh CD27 low memory-like NK cells that prevent melanoma lung metastasis. SITC 38th Annual Meeting (San Diego Convention Center, San Diego, Nov 1-5, 2023)