工学研究院 業績一覧

マイクロ流路グループ

 

研究プロジェクト 第3期 業績一覧（2019年～2023年）

＜原著論文＞
  1. Sato Y, Hashiba K, Sasaki K, Maeki M, Tokeshi M, Harashima H. Understanding structure-activity relationships of pH-sensitive cationic lipids facilitates the rational identification of promising lipid nanoparticles for delivering siRNAs in vivo. J Control Release 295:140-152 (2019). doi: 10.1016/ j.jconrel.2019.01.001
  2. Hibino M, Yamada Y, Fujishita N, Sato Y, Maeki M, Tokeshi M, Harashima H. The use of a microfluidic device to encapsulate a poorly water-soluble drug CoQ10 in lipid nanoparticles and an attempt to regulate intracellular trafficking to reach mitochondria. J Pharm Sci 108: 2668-2676 (2019) doi: 10.1016/ j.xphs.2019.04.001
  3. Nishiyama K, Sugiura K, Kaji N, Tokeshi M, Baba Y. Development of a microdevice facile analysis of theophylline in whole blood by a cloned enzyme donor immunoassay. Lab Chip. 19: 233-240 (2019). Doi: 10.1039/c8lc01105b
  4. Nishiyama K, Kasama T, Nakamata S, Ishikawa K, Onoshima D, Yukawa H, Maeki M, Ishida A, Tani H, Baba Y, Tokeshi M. Ultrasensitive detection of disease biomarkers using an immuno-wall device with enzymatic amplification. Analyst 144:4589-4595 (2019). doi: 10.1039/c9an00480g
  5. Kenia C.R, Nishiyama K, Maeki M, Ishida A, Tani H, Kasawa T, Baba Y, Tokeshi M. Rapid, sensitive, and selective detection of H5 hemagglutinin from avian influenza virus using an immunowall device. ACS Omega 4: 16683-16688 (2019). doi: 10.1021/acsomega.9b02788
  6. Sato Y, Okabe N, Note Y, Hashiba K, Maeki M, Tokeshi M, Harashima H. Hydrophobic scaffolds of pH-sensitive cationic lipids contribute to miscibility with phospholipids and improve the efficiency of delivering short interfering RNA by small-sized lipid nanoparticles. Acta Biomater 102: 341-350 (2020). doi: 10.1016/j.actbio.2019.11.022
  7. Nakamura T, Kawai M, Sato Y, Maeki M, Tokeshi M, Harashima H. The effect of size and charge of lipid nanoparticles prepared by microfluidic mixing on their lymph node transitivity and distribution. Mol Pharm 17: 944-953 (2020). doi: 10.1021/acs.molpharmaceut.9b01182
  8. Komatsu T, Maeki M, Ishida A, Tani H, Tokeshi M. Paper-based device for the facile colorimetric determination of lithium ions in human whole blood. ACS Sens 5: 1287-1294 (2020). doi: 1021/acssensors.9b02218
  9. Nishiyama K, Takeda Y, Maeki M, Ishida A, Tani H, Shigemura K, Hibara A, Yonezawa Y, Imai K, Ogawa H, Tokeshi M. Rapid detection of anti-H5 avian influenza virus antibody by fluorescence polarization immunoassay using a portable fluorescence polarization analyzer. Sens Actuators B 316: 128160 (2020). doi: 1016/j.snb.2020.128160
  10. Kimura N, Maeki M, Sato Y, Ishida A, Tani H, Harashima H, Tokeshi M. Development of a microfluidic-based post-treatment process for size-controlled lipid nanoparticles and application to siRNA delivery. ACS Appl Mater Interfaces. 12: 34011-34020 (2020). doi: 1021/acsami.0c05489.
  11. Fukuyama M, Nakamura A, Nishiyama K, Imai A, Tokeshi M, Shigemura K, Hibara A. Noncompetitive fluorescence polarization immunoassay for protein determination. Anal Chem 92: 14393-14397 (2020). doi: 1021/acs.analchem.0c02300
  12. Hashiba A, Toyooka M, Sato Y, Maeki M, Tokeshi M, Harashima H. The use of design of experiments with multiple responses to determine optimal formulations for in vivo hepatic mRNA delivery. J Control Release 327: 467-476 (2020). doi: 1016/j.jconrel.2020.08.031
  13. Yogo N, Hase T, Kasama T, Nishiyama K, Ozawa N, Hatta T, Shibata H, Sato M, Komeda K, Kawabe N, Matsuoka K, Fengshi Chen-Yoshikawa T, Kaji N, Tokeshi M, Baba Y, Hasegawa Y. Development of an immuno-wall device for the rapid and sensitive detection EGFR mutations in tumor tissues resected from lung cancer patients. PLOS ONE 15: e0241422 (2020). doi: 1371/journal.pone.0241422
  14. Wang Y, Quinsaat JEQ, Ono T, Maeki M, Tokeshi M, Isono T, Tajima K, Satoh T, Sato S, Miura Y, Yamamoto T. Enhanced dispersion stability of gold nanoparticles by the physisorption of cyclic poly(ethylene glycol). Nature Commun 11: 6089 (2020). doi: 10.1038/s41467-020-19947-8
  15. Komatsu T, Sato Y, Maeki M, Ishida A, Tani H, Tokeshi M. Rapid, sensitive universal paper-based device enhances competitive immunoassays of small molecules. Anal Chim Acta 1144: 85-95 (2021). doi: 1016/j.aca.2020.12.003
  16. Suzuki Y, Onuma H, Sato R, Sato Y, Hashiba A, Maeki M, Tokeshi M, Kayesh MEH, Kohara M, Tsukiyama-Kohara K, Harashima H. Lipid nanoparticles loaded with ribonucleoprotein-oligonucleotide complexes synthesized using a microfluidic device exhibit robust genome editing and hepatitis B virus inhibition, J Control Release 330: 61-71 (2021). doi: 1016/j.jconrel.2020.12.013
  17. Nishiyama K, Fukuyama M, Maeki M, Ishida A, Tani H, Hibara A, Tokeshi M. One-step non-competitive fluorescence polarization immunoassay based on a Fab fragment for C-reactive protein quantification. Sens Actuators B 326: 128982 (2021). doi: 1016/j.snb.2020.128982
  18. Kimura N, Maeki M, Sasaki K. Sato Y, Ishida A, Tani H, Harashima H, Tokeshi M. Three-dimensional, symmetrically assembled microfluidic device for lipid nanoparticle production. RSC Adv 11: 1430-1439 (2021). doi: 10.1039/d0ra08826a
  19. Kimura N, Maeki M, Ishida A, Tani H, Tokeshi M. One step production of highly biocompatible size-controlled non-cationic exosome-like nanoparticles for RNA delivery using a microfluidic device. ACS Appl Bio Mater 4: 1783-1793 (2021). doi: 10.1021/acsabm.0c01519.
  20. Nishiyama K, Takeda Y, Takahashi K, Fukuyama M, Maeki M, Ishida A, Tani H, Shigemura K, Hibara A, Ogawa H, Tokeshi M. Non-competitive fluorescence polarization immunoassay for detection of H5 avian influenza virus using a portable analyzer. Anal Bioanal Chem 413: 4619-4623 (2021). doi: 1007/s00216-021-03193-y
  21. Tanaka H, Tanaka N, Sakurai Y, Yoshida Y, Inoue T, Tamagawa S, Nakai Y, Tange K, Yoshioka H, Maeki M, Tokeshi M, Akita H. Delivery of oligonucleotides using a self-degradable lipid-like material, Pharmaceutics 13: 544 (2021). doi: 3390/pharmaceutics13040544
  22. Nishiyama K, Takahashi K, Fukuyama M, Kasuya M, Imai A, Usukura T, Maishi N, Maeki M, Ishida A, Tani H, Hida K, Shigemura K, Hibara A, Tokeshi M. Facile and rapid detection of SARS-CoV-2 antibody based on a nonconpetitive fluorescence polarization immunoassay in human serum samples. Biosens Bioelectron 190: 113414 (2021). doi: 10.1016/j.bios.2021.113414
  23. Komatsu T, Tokeshi M, Fan SK. Determination of blood lithium-ion concentration using digital microfluidic whole-blood separation and preloaded paper sensors. Biosens Bioelectron 195: 113631 (2022). doi: 1016/j.bios.2021.113631
  24. Nishiyama K, Mizukami R, Kuki S, Ishida A, Chida J, Kido H, Maeki M, Tani H, Tokeshi M. Electrochemical enzyme-based blood ATP and lactate sensor for a rapid and straightforward evaluation of illness severity. Biosens Bioelectron 198: 113832 (2022). doi: 1016/j.bios.2021.113822
  25. Oziri OJ, Wang Y, Watanabe T, Uno S, Maeki M, Tokeshi M, Isono T, Tajima K, Satoh T, Sato S, Miura Y, Yamamoto T. PEGylation of silver nanoparticles by physisorption of cyclic poly(ethylene glycol) for enhanced dispersion stability, antimicrobial activity, and cytotoxicity. Nanoscale Adv 4: 532-545 (2022). doi: 1039/d1na00720c
  26. Maeki M, Okada Y, Uno S, Niwa A, Ishida A, Tani H, Tokeshi M. Production of siRNA-loaded lipid nanoparticles using a microfluidc device. J Vis Exp 181: e62999 (2022). doi: 10.3791/62999
  27. Oziri OJ, Maeki M, Tokeshi M, Isono T, Tajima K, Satoh T, Sato S, Yamamoto T. Topology-dependent interaction of cyclic poly(ethylene glycol) complexed with gold nanoparticles against bovine serum albumin for a colorimetric change. Langmuir 38: 5286-5295 (2022). doi: 1021/acs.langmuir.1c03027
  28. Matsuura-Sawada Y, Maeki M, Nishioka T, Niwa A, Yamaguchi J, Mizoguchi M, Wada K, Tokeshi M. Microfluidic Device-enabled mass production of lipid-based nanoparticles for applications in nanomedicine and cosmetics. ACS Appl Nano Mater 5: 7867-7876 (2022). doi: 10.1021/acsanm.2c00886
  29. Okuda K, Sato Y, Kazuki Iwakawa, Sasaki K, Nana Okabe, Maeki M, Tokeshi M, Harashima H. On the size-regulation of RNA-loaded lipid nanoparticles synthesized by microfluidic device. J Control Release 348: 648-659 (2022) doi: 10.1016/j.jconrel.2022.06.017
  30. Yi B, Maeki M, Ishida A, Tani H, Tokeshi M. Preparation of size-tunable sub-200 nm PLGA-based nanoparticles with a wide size range using a microfluidic device. PLOS ONE 17: e0271050 (2022). doi: 10.1371/journal.pone.0271050
  31. Takahashi K, Chida S, Suwatthanarak T, Iida M, Zhang M, Fukuyama M, Maeki M, Ishida A, Tani H, Yasui T, Baba Y, Hibara A, Okochi M, Tokeshi M. Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer. Lab Chip 22: 2971-2977 (2022). doi: 10.1039/D2LC00224H
  32. Yi B, Maeki M, Ishida A, Tani H, Tokeshi M. Effect of organic solvent on a production of PLGA-based drug-loaded nanoparticles using a microfluidic device. ACS Omega 7: 33079-33086 (2022). doi.org/10.1021/acsomega.2c03137
  33. Kubota F, Satrialdi, Takano Y, Maeki M, Tokeshi M, Harashima H, Yamada Y. Fine-tuning the encapsulation of a photosensitizer in nanoparticles reveals the relationship between internal structure and phototherapeutic effects. J Biophotonics 16: e202200119 (2023). DOI: 10.1002/jbio.202200119.
  34. Maeki M, Okada Y, Uno S, Sugiura K, Suzuki Y, Sato Y, Ando M, Yamazaki H, Takeuchi M, Ishida A, Tani H, Harashima H, Tokeshi M. Mass production system for RNA-loaded lipid nanoparticles using pilling up microfluidic devices. Appl Mater Today 31: 101754 (2023). doi: 1016/j.apmt.2023.101754
  35. Matsuura-Sawada Y, Uno S, Maeki M, Wada K, Tokeshi M. Controlling lamellarity and physicochemical properties of liposomes prepared using a microfluidic device. Biomater Sci 11: 2419-2426 (2023). doi: 1039/D2BM01703B
  36. Hibino M, Maeki M, Tokeshi M, Ishitsuka Y, Harashima H, Yamada Y*, A system that delivers an antioxidant to mitochondria for the treatment of drug-induced liver injury. Sci Rep 13: 6961 (2023). DOI: 10.1038/s41598-023-33893-7.
  37. Ito K, Furukawa H, Inaba H, Oshima S, Kametani Y, Maeki M, Tokeshi M, Huang X, Kabayama K, Manabe Y, Fukase K, Matsuura K. Antigen/adjuvant-displaying enveloped viral replica as a self-adjuvanting anti-breast-cancer vaccine candidate. J Am Chem Soc 145: 15838-15847 (2023). doi: 1021/jacs.3c02679
  38. Kametani Y, Ito R, Ohshima S, Manabe Y, Ohno Y, Shimizu T, Yamada S, Katano N, Kirigaya D, Ito K, Matsumoto T, Tsuda B, Kashiwagi H, Goto Y, Yasuda A, Maeki M, Tokeshi M, Seki T, Fukase K, Mikami M, Ando K, Ishimoto H, Shiina T. Construction of the systemic anticancer immune environment in tumor-bearing humanized mouse by using liposome-encapsulated anti-programmed death ligand 1 antibody-conjugated progesterone. Front Immunol 14: 1173728 (2023). doi: 10.3389/fimmu.2023.1173728
  39. Oyama R, Ishigame H, Tanaka H, Tateshita N, Itazawa M, Imai R, Nishiumi N, Kishikawa J, Kato T, Nishikawa Y, Anindita J, Maeki M, Tokeshi M, Tange K, Naka Y, Sakurai Y, Okada T, Akita H. An ionizable llipid material with a vitamin E scaffold as an mRNA vaccine platform for efficient cytotoxic T cell responses. ACS Nano 17: 18758-18774. doi: 10.1021/acsnano.3c02251

 

＜総説論文（英語）＞
  1. Maeki M, Uno S, Niwa A, Okada Y, Tokeshi M. Microfluidic technologies and devices for lipid nanoparticle-based RNA delivery. J Control Release 344: 80-96 (2022). doi: 10.1016/j.jconrel.2022.02.017
  2. Shalaby AA, Tsao C.-W, Ishida A, Tokeshi M. Microfluidic paper-based analytical devices for cancer diagnosis. Sens. Actuators B 379: 133243 (2023). doi: 1016/j.snb.2022.133243

 

 

＜総説論文（日本語）＞

 

 

 

＜著書（英語）＞
  1. Kimura N, Maeki M, & Tokeshi M, “Microfabrication and Microfluidic Devices for Drug Delivery”, Microfluidics for pharmaceutical applications, From nano/micro systems fabrication to controlled drug delivery (Helder A. Santos, Dongfei Liu, & Hongbo Zhang), p123-136, 2019, Elsevier

 

 

＜著書（日本語）＞

 

 

 

＜記事＞
  1. 「ライラックファーマ株式会社との化粧品用素材事業に関する契約締結のお知らせ」　株式会社日本触媒　HP 2019年3月5日（火）
  2. 「日本触媒、化粧品材拡大」　日刊工業新聞　2019年3月5日（火）

 

 

＜その他＞

 

 

 

＜受賞＞

 

 

 

＜招待講演＞
  1. Maeki M, “Precise size tuning of lipid nanoparticles using baffle mixer devices, Pittcon Conference & Expo (Mar 18, Pennsylvania, USA)
  2. 真栄城正寿　「脂質ナノ粒子の超高速時間分解小角散乱測定」　北海道大学-高エネルギー加速器研究機構 第9回 連携シンポジウム　2019年3月27日　つくば

 

 

＜国際学会発表（一般演題）＞
  1. Kimura N, Maeki M, Note Y, Sato Y, Ishida A, Tani H, Harashima H, Tokeshi M, “Development of microfluidic devices for lipid nanoparticles preparation and its application for drug delivery systems” 2019 Global Young Scientists Summit (Jan 20-25, Singapore)

 

 

＜国内学会発表（一般演題）＞

 

 

 

＜知的財産＞